CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

Traditionally, generation of new plants with improved or desirable features has relied on
laborious and time-consuming breeding techniques. Genome-editing technologies have led …

Genome editing is an approach in which a specific target DNA sequence of the genome is
altered by adding, removing, or replacing DNA bases. Artificially engineered hybrid …

CRISPR/Cas mediated genome editing is a revolutionary approach for manipulating the
plant genome. However, the success of this technology is highly dependent on selection of a …

The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse
organisms including plants, and has quickly evolved into a suite of versatile tools for …

Background To accelerate the application of the CRISPR/Cas9 (clustered regularly
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …

The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-
associated nuclease 9) system is a versatile tool for genome engineering that uses a guide …

Since its first appearance, CRISPR–Cas9 has been developed extensively as a
programmable genome-editing tool, opening a new era in plant genome engineering …

The increasing burden of the world population on agriculture requires the development of
more robust crops. Dissecting the basic biology that underlies plant development and stress …

The CRISPR/Cas9 genome engineering system has ignited and swept through the scientific
community like wildfire. Owing largely to its efficiency, specificity, and flexibility, the …