A comprehensive analysis of human dental pulp cell spheroids in a three-dimensional pellet culture system

S Zhang, P Buttler-Buecher, B Denecke… - Archives of oral …, 2018 - Elsevier
Abstract Objective Three-dimensional (3D) cell culture methods are of high importance to
studies of biological processes. This is particularly the case with spheroid cultures, which …

Three-dimensional spheroid culture promotes odonto/osteoblastic differentiation of dental pulp cells

M Yamamoto, N Kawashima, N Takashino… - Archives of Oral …, 2014 - Elsevier
Abstract Objective Three-dimensional (3D) spheroid culture is a method for creating 3D
aggregations of cells and their extracellular matrix without a scaffold mimicking the actual …

In vitro characterization of dental pulp stem cells cultured in two microsphere-forming culture plates

NU Bu, HS Lee, BN Lee, YC Hwang, SY Kim… - Journal of Clinical …, 2020 - mdpi.com
Various three-dimensional (3D) culture methods have been introduced to overcome the
limitations of in vitro culture and mimic in vivo conditions. This study aimed to evaluate two …

Three-dimensional sphere-forming cells are unique multipotent cell population in dental pulp cells

SH Lee, A Inaba, N Mohindroo, D Ganesh… - Journal of …, 2017 - Elsevier
Abstract Introduction Mesenchymal stem cells (MSCs) are typically cultured as adherent
monolayer using a conventional tissue culture technique. However, this technique …

[HTML][HTML] 3D spheroid formation using BMP-loaded microparticles enhances odontoblastic differentiation of human dental pulp stem cells

TJ Min, MJ Kim, KJ Kang, YJ Jeoung, SH Oh… - Stem Cells …, 2021 - hindawi.com
Human dental pulp stem cells (hDPSCs) are the primary cells responsible for dentin
regeneration. Typically, in order to allow for odontoblastic differentiation, hDPSCs are …

Dentin-like tissue formation and biomineralization by multicellular human pulp cell spheres in vitro

J Neunzehn, MT Weber, G Wittenburg, G Lauer… - Head & Face …, 2014 - Springer
Introduction Maintaining or regenerating a vital pulp is a preferable goal in current
endodontic research. In this study, human dental pulp cell aggregates (spheres) were …

Characterization of human dental pulp cells‐derived spheroids in serum‐free medium: Stem cells in the core

L Xiao, T Tsutsui - Journal of cellular biochemistry, 2013 - Wiley Online Library
Spheroid models have led to an increased understanding of differentiation, tissue
organization and homeostasis. In the present study, we have observed that under a serum …

Three-dimensional spheroid culture of dental pulp-derived stromal cells enhance their biological and regenerative properties for potential therapeutic applications

S Raik, P Sharma, S Kumar, V Rattan, A Das… - … International Journal of …, 2023 - Elsevier
Mesenchymal stem/stromal cell (MSC) spheroids generated in a three-dimensional (3D)
culture system serve as a surrogate model that maintain stem cell characteristics since these …

Ultrastructural characterization of human gingival fibroblasts in 3D culture

SL Alfonso García, LM Mira Uribe, S Castaño López… - Cells, 2022 - mdpi.com
Cell spheroids are applied in various fields of research, such as the fabrication of three-
dimensional artificial tissues in vitro, disease modeling, stem cell research, regenerative …

In Vitro Bone Differentiation of 3D Microsphere from Dental Pulp-Mesenchymal Stem Cells

I Gaitán-Salvatella, P González-Alva, JJ Montesinos… - Bioengineering, 2023 - mdpi.com
Bone defects lead to the structural loss of normal architecture, and those in the field of bone
tissue engineering are searching for new alternatives to aid bone regeneration. Dental pulp …