Libraries constructed in bacterial artificial chromosome (BAC) vectors have become the
choice for clone sets in high throughput genomic sequencing projects primarily because of …

End sequences from bacterial artificial chromosomes (BACs) provide highly specific
sequence markers in large-scale sequencing projects. To date, we have generated> …

Large-insert genomic DNA libraries are based on the Escherichia coli F factor, a low-copy
plasmid that exits in a supercoiled circular form in the host cells. These libraries are used to …

Bacterial vectors containing large inserts of genomic DNA are now the standard substrates
for large-scale genomic sequencing. Long overlaps between some clones lead to …

DNA cloning, especially large DNA cloning, is the first step in contemporary complex
genome analysis. Cloning technology of high-molecular-weight DNA has been developed …

Abstract Applications that use Bacterial Artificial Chromosome (BAC) libraries often require
paired-end sequences and knowledge of the physical location of each clone in plates. To …

We have constructed an arrayed human genomic BAC library with approximately 4×
coverage that is represented by 96,000 BAC clones with average insert size of nearly 140 …

The bacterial artificial chromosome (BAC)(1) is now the most widely utilized vector system to
construct large-insert genomic libraries for genome analysis. It has the following advantages …

A significant bottleneck in large-scale DNA sequencing projects is the creation of high-
quality random libraries suitable for sequencing. We have utilized an adaptor-based strategy …

A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large
average insert size (178 kb) has been constructed to provide the intermediate substrate for …