A scanning microspectrophotometer controlled by a small digital computer is used to
produce data which is analysed using a larger, remote digital computer. Programs have …

Most commercial microphotometers have been designed to measure spectrographic plates,
recording transmissions substantially along the major axis of the plate by means either of a …

ROBERT C. MELLORS 399 to detect in cervical smears optical differences between normal
cells and cells which have abnormal features suggestive of but not conclusive for cancer …

A microspectrophotometer of increased sensitivity has been constructed to rapidly record
absorption spectra in a single sweep at relatively low light levels from the ultraviolet through …

The calculations required for two-wavelength measurements are time consuming and
laborious. In order to circumvent this limitation of the method, a set of tables which combined …

Publisher Summary This chapter discusses quantitative microscopical techniques for single
cells and describes the techniques in which a microscope with ancillary equipment is used …

The two-wavelength method of microspectrophotometry corrects for distributional error and
measures the amount of absorbing material by taking advantage of certain spectral …

Nearly all quantitative, absorption, microspectrophotometry has been done using smear
preparations in which the cells are isolated against a relatively clear background. When …

A photoelectric method for the rapid determination of the total absorption of biological cells is
described. The image of the cell, formed by a microscope, is scanned by a mechanically …