Physical and Functional Analysis of the Prokaryotic Enhancer of the σ54-promoters of the TOL Plasmid ofPseudomonas putida

J Pérez-Martı́n, V de Lorenzo - Journal of molecular biology, 1996 - Elsevier
The physical and the functional organization of the upstreamcis-acting sequence that
controls at a distance the transcriptional activity ofPuandPs, the two σ54-dependent …

Physical organization of the upper pathway operon promoter of the Pseudomonas TOL plasmid. Sequence and positional requirements for XylR-dependent activation …

MA Abril, JL Ramos - Molecular and General Genetics MGG, 1993 - Springer
The upper pathway operon of the Pseudomonas putida TOL plasmid belongs to the− 12/-24
class of promoters. These promoters exhibit three regions critical for regulated transcription …

Activation of the Pseudomonas TOL plasmid upper pathway operon. Identification of binding sites for the positive regulator XylR and for integration host factor protein

MA Abril, M Buck, JL Ramos - Journal of Biological Chemistry, 1991 - ASBMB
Expression of the Pseudomonas putida TOL plasmid upper pathway operon requires a
promoter that belongs to the-12/-24 class. Stimulation of transcription from this promoter is …

The sigma 54-dependent promoter Ps of the TOL plasmid of Pseudomonas putida requires HU for transcriptional activation in vivo by XylR

J Pérez-Martín, V de Lorenzo - Journal of bacteriology, 1995 - Am Soc Microbiol
In the presence of toluene and xylenes, the sigma 54-dependent Ps promoter of the TOL
(toluene biodegradation) plasmid pWW0 of Pseudomonas putida is activated at a distance …

Activation and Repression of Transcription at the Double Tandem Divergent Promoters for the xylR and xylS Genes of the TOL Plasmid of Pseudomonas putida

S Marqués, MT Gallegos, M Manzanera… - Journal of …, 1998 - Am Soc Microbiol
The xylR and xylS genes are divergent and control transcription of the TOL plasmid
catabolic pathways for toluene metabolism. Four promoters are found in the 300-bp …

In vitro activities of an N-terminal truncated form of XylR, a σ54-dependent transcriptional activator of Pseudomonas putida

J Pérez-Martı́n, V de Lorenzo - Journal of molecular biology, 1996 - Elsevier
A truncated derivative of the XylR protein, which is able to constitutively activate the σ54-
dependentPupromoter of the TOL (toluene biodegradation) plasmid ofPseudomonas putida …

Promoter‐upstream activator sequences are required for expression of the xylS gene and upper‐pathway operon on the Pseudomonas TOL plasmid

A Holtel, MA Abril, S Marques, KN Timmis… - Molecular …, 1990 - Wiley Online Library
Stimulation of transcription from the Pseudomonas TOL plasmid xylS gene promoter (Ps)
and the upperpathway operon promoter (Pu) is dependent on the positive regulator protein …

Activation of the toluene‐responsive regulator XylR causes a transcriptional switch between σ54 and σ70 promoters at the divergent Pr/Ps region of the TOL plasmid

G Bertoni, S Marqués, V De Lorenzo - Molecular microbiology, 1998 - Wiley Online Library
The mechanism by which XylR, the toluene‐responsive activator of the σ54‐dependent Pu
and Ps promoters of the Pseudomonas TOL plasmid pWW0, downregulates its own σ70 …

Genetic evidence of separate repressor and activator activities of the XylR regulator of the TOL plasmid, pWWO, of Pseudomonas putida

G Bertoni, J Perez‐Martfn… - Molecular …, 1997 - Wiley Online Library
The XylR protein encoded by pWWO, the TOL (toluene biodegradation) plasmid of
Pseudomonas putida, activates at a distance the transcription of Pu and Ps, which are the …

Transcriptional control of the Pseudomonas putida TOL plasmid catabolic pathways

S Marqués, JL Ramos - Molecular microbiology, 1993 - Wiley Online Library
TOL plasmid pWWO of Pseudomonas putida contains two operons that specify a pathway
for the degradation of aromatic hydrocarbons. The upper pathway operon encodes the …