Role of σs in transcription from the positively controlled Pm promoter of the TOL plasmid of Pseudomonas putida

S Marqués, MT Gallegos, JL Ramos - Molecular microbiology, 1995 - Wiley Online Library
Transcription from the TOL plasmid Pm promoter is dependent on the XylS regulator
activated by benzoate effectors. We analysed transcription from Pm in several backgrounds …

Transcriptional control of the multiple catabolic pathways encoded on the TOL plasmid pWW53 of Pseudomonas putida MT53

MT Gallegos, PA Williams, JL Ramos - Journal of bacteriology, 1997 - Am Soc Microbiol
The TOL plasmid pWW53 encodes a catabolic pathway for the metabolism of toluene. It
bears an upper-pathway operon for the oxidation of toluene to benzoate and a copy of the …

Expression of the TOL plasmid xylS gene in Pseudomonas putida occurs from a alpha 70-dependent promoter or from alpha 70-and alpha 54-dependent tandem …

MT Gallegos, S Marqués, JL Ramos - Journal of bacteriology, 1996 - Am Soc Microbiol
Growth of Pseudomonas putida (pWWO) on alkylbenzoates requires the expression of the
meta pathway operon, which is mediated by the XylS protein after binding of a benzoate …

Transient XylR binding to the UAS of the Pseudomonas putida σ54 promoter Pu revealed with high intensity UV footprinting in vivo

M Valls, V de Lorenzo - Nucleic acids research, 2003 - academic.oup.com
The binding of the transcriptional regulator XylR to its cognate upstream activating
sequences (UAS) of the σ54‐dependent promoter Pu of Pseudomonas putida has been …

Visualization of DNA–protein intermediates during activation of the Pu promoter of the TOL plasmid of Pseudomonas putida

J Garmendia, V de Lorenzo - Microbiology, 2000 - microbiologyresearch.org
The ATP-dependent multimerization process undergone by the σ54-dependent activator
XylR of the TOL plasmid pWW0 of Pseudomonas putida when bound to the upstream …

Transcriptional induction kinetics from the promoters of the catabolic pathways of TOL plasmid pWW0 of Pseudomonas putida for metabolism of aromatics

S Marqués, A Holtel, KN Timmis… - Journal of bacteriology, 1994 - Am Soc Microbiol
We determined, under several growth conditions, the kinetics of mRNA synthesis from the
four Pseudomonas putida pWW0 plasmid promoters involved in degradation of xylenes and …

Upstream binding sequences of the XyIR activator protein and integration host factor in the xylS gene promoter region of the Pseudomonas TOL plasmid

A Holtel, KN Timmis, JL Ramos - Nucleic acids research, 1992 - academic.oup.com
The xylR and xylS genes, which encode the positive regulators of the TOL plasmid catabolic
pathways, are adjacent genes on the TOL plasmid and are transcribed from divergent …

Integration host factor suppresses promiscuous activation of the sigma 54-dependent promoter Pu of Pseudomonas putida.

J Pérez-Martín, V De Lorenzo - Proceedings of the National …, 1995 - National Acad Sciences
In the presence of m-xylene, the Pu promoter of the TOL plasmid of Pseudomonas putida is
activated by the prokaryotic enhancer-binding protein XylR. The intervening DNA segment …

Early and late responses of TOL promoters to pathway inducers: identification of postexponential promoters in Pseudomonas putida with lacZ-tet bicistronic reporters

V De Lorenzo, I Cases, M Herrero… - Journal of …, 1993 - Am Soc Microbiol
Transcriptional lacZ fusions to the Pu and Pm promoters of the TOL (toluene degradation)
plasmid inserted in monocopy in the chromosome of Pseudomonas putida showed a very …

Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains

S Fernández, V de Lorenzo… - Molecular …, 1995 - Wiley Online Library
In the presence of toluene, xylenes and other structural analogues, the regulatory protein
XylR, of the family of transcriptional regulators which act in concert with the σ54 factor …