Multiplex PCR: optimization and application in diagnostic virology

EM Elnifro, AM Ashshi, RJ Cooper… - Clinical microbiology …, 2000 - Am Soc Microbiol
SUMMARY PCR has revolutionized the field of infectious disease diagnosis. To overcome
the inherent disadvantage of cost and to improve the diagnostic capacity of the test …

Real-time PCR in virology

IM Mackay, KE Arden, A Nitsche - Nucleic acids research, 2002 - academic.oup.com
The use of the polymerase chain reaction (PCR) in molecular diagnostics has increased to
the point where it is now accepted as the gold standard for detecting nucleic acids from a …

Using multiplex real time PCR in order to streamline a routine diagnostic service

RN Gunson, S Bennett, A Maclean… - Journal of Clinical …, 2008 - Elsevier
An increasing number of virology laboratories are now utilising in house real time PCR
assays as the frontline diagnostic tests. As the number of tests on offer increases the natural …

Detection and monitoring of virus infections by real-time PCR

F Watzinger, K Ebner, T Lion - Molecular aspects of medicine, 2006 - Elsevier
The employment of polymerase chain reaction (PCR) techniques for virus detection and
quantification offers the advantages of high sensitivity and reproducibility, combined with an …

Practical experience of high throughput real time PCR in the routine diagnostic virology setting

RN Gunson, TC Collins, WF Carman - Journal of Clinical Virology, 2006 - Elsevier
The advent of PCR has transformed the utility of the virus diagnostic laboratory. In
comparison to traditional gel based PCR assays, real time PCR offers increased sensitivity …

[PDF][PDF] Quantitative PCR and RT-PCR in virology

M Clementi, S Menzo, P Bagnarelli… - PCR methods and …, 1993 - academia.edu
In the last few years, molecular hybridization methods have been used extensively in basic
and applied virology because of their technical flexibility and high specificity. Using these …

Molecular diagnosis of medical viruses

RM Ratcliff, G Chang, TW Kok, TP Sloots - Current issues in molecular …, 2007 - mdpi.com
The diagnosis of infectious diseases has been revolutionized by the development of
molecular techniques, foremost with the applications of the polymerase chain reaction …

Real-time PCR in clinical microbiology: applications for routine laboratory testing

MJ Espy, JR Uhl, LM Sloan… - Clinical microbiology …, 2006 - Am Soc Microbiol
Real-time PCR has revolutionized the way clinical microbiology laboratories diagnose many
human microbial infections. This testing method combines PCR chemistry with fluorescent …

[HTML][HTML] Real-time PCR in the microbiology laboratory

IM Mackay - Clinical microbiology and infection, 2004 - Elsevier
Use of PCR in the field of molecular diagnostics has increased to the point where it is now
accepted as the standard method for detecting nucleic acids from a number of sample and …

Application of polymerase chain reaction to the diagnosis of infectious diseases

DN Fredricks, DA Relman - Clinical infectious diseases, 1999 - JSTOR
PCR and other sequence-based microbial detection methods, once considered to be only
research tools, are being used increasingly in the clinical microbiology laboratory. As this …