We have developed a real-time reverse transcription-PCR (RT-PCR) method to detect 13
respiratory viruses: influenza virus A and B; respiratory syncytial virus (RSV) subgroup A and …

To evaluate pathogens in pediatric inpatients with community-acquired pneumonia (CAP),
an Acute Respiratory Diseases Study Group organized by ten Japanese medical institutions …

Objectives To develop and evaluate a novel method for simultaneous identification of 12
potential bacterial pathogens in children with community‐acquired pneumonia. Methods A …

Abstract Children (n= 117; mean age 2.4±2.9 years) were diagnosed as having community-
acquired pneumonia (CAP) using clinical symptoms, chest X-rays, and hematological data …

Reverse transcription-PCR (RT-PCR) is a sensitive method for detection of RNA virus
nucleic acid sequences in clinical respiratory specimens. Previous studies have focused on …

Respiratory viruses are a common cause of respiratory tract infection (RTI), particularly in
neonates and children. Rapid and accurate diagnosis of viral infections could improve …

ABSTRACT A reverse transcription-PCR (RT-PCR) assay based on automated fluorescent
capillary electrophoresis and GeneScan software analysis was developed to detect six …

Several studies examining the incidence of viral infection in childhood community‐acquired
pneumonia (CAP) utilizing polymerase chain reaction (PCR) or real‐time PCR methods …

A 4-tube multiplex RT-PCR (mRT-PCR), which showed higher sensitivity over conventional
methods, was previously developed for the diagnosis of 14 viral pathogens of the respiratory …

Background: Respiratory viruses are the main cause of acute respiratory tract infection (ARI)
in children. Real-time polymerase chain reaction (PCR) technology is highly practicable for …