Engineered thermostable fungal cellulases exhibit efficient synergistic cellulose hydrolysis at elevated temperatures

DL Trudeau, TM Lee, FH Arnold - Biotechnology and …, 2014 - Wiley Online Library
ABSTRACT A major obstacle to using widely available and low‐cost lignocellulosic
feedstocks to produce renewable fuels and chemicals is the high cost and low efficiency of …

Characterization and efficient production of a thermostable, halostable and organic solvent-stable cellulase from an oil reservoir

T Zhu, R Li, J Sun, Y Cui, B Wu - International journal of biological …, 2020 - Elsevier
The manufacture of biofuels from cellulose is regarded as one of practicable strategies to
meet increasing energy demand and alleviate environmental issues. Cellulases, which play …

A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities

Y Zhou, X Wang, W Wei, J Xu, W Wang, Z Xie… - Biotechnology for …, 2016 - Springer
Background Cellulose, an abundant and renewable polysaccharides, constitutes the largest
resource for bioconversion of biofuels. Plant polysaccharides hydrolysis is catalyzed by …

Improved Activity of a Thermophilic Cellulase, Cel5A, from Thermotoga maritima on Ionic Liquid Pretreated Switchgrass

Z Chen, JH Pereira, H Liu, HM Tran, NSY Hsu… - PLoS …, 2013 - journals.plos.org
Ionic liquid pretreatment of biomass has been shown to greatly reduce the recalcitrance of
lignocellulosic biomass, resulting in improved sugar yields after enzymatic saccharification …

Structure and function of Humicola insolens family 6 cellulases: structure of the endoglucanase, Cel6B, at 1.6 Å resolution

GJ Davies, AM Brzozowski, M Dauter… - Biochemical …, 2000 - portlandpress.com
Cellulases are traditionally classified as either endoglucanases or cellobiohydrolases on the
basis of their respective catalytic activities on crystalline cellulose, which is generally …

Glycosylation is vital for industrial performance of hyperactive cellulases

D Chung, NS Sarai, BC Knott, N Hengge… - ACS Sustainable …, 2019 - ACS Publications
In the terrestrial biosphere, biomass deconstruction is conducted by microbes employing a
variety of complementary strategies, many of which remain to be discovered. Moreover, the …

Improved thermostability of Clostridium thermocellum endoglucanase Cel8A by using consensus-guided mutagenesis

M Anbar, O Gul, R Lamed, UO Sezerman… - Applied and …, 2012 - Am Soc Microbiol
The use of thermostable cellulases is advantageous for the breakdown of lignocellulosic
biomass toward the commercial production of biofuels. Previously, we have demonstrated …

Revisiting cellulase production and redefining current strategies based on major challenges

RC Kuhad, D Deswal, S Sharma… - … and Sustainable Energy …, 2016 - Elsevier
Lignocellulosic biomass has been considered as an important and sustainable source of
renewable energy. Cellulose constitutes the major component of the lignocellulosic biomass …

Expression and characterization of a novel metagenome-derived cellulase Exo2b and its application to improve cellulase activity in Trichoderma reesei

A Geng, G Zou, X Yan, Q Wang, J Zhang, F Liu… - Applied microbiology …, 2012 - Springer
A metagenomic fosmid library containing 1× 10 5 clones was constructed from a biogas
digester fed with pig ordure and rice straw. In total, 121 clones with activity of 4 …

Protein engineering of cellulases

AS Bommarius, M Sohn, Y Kang, JH Lee… - Current opinion in …, 2014 - Elsevier
Highlights•Scarce experimental data drive the importance of computational results in
cellulase engineering.•More and more new helper proteins will aid cellulases in the overall …