The aims of high‐throughput (HTP) protein production systems are to obtain well‐expressed
and highly soluble proteins, which are preferred candidates for use in structure–function …

Despite the large body of knowledge accumulated on recombinant protein expression,
production, primarily of eukaryotic proteins, remains a challenge. The biggest obstacle is in …

For future structural and functional genomics programs new tools will be required. The
implementation of high-throughput (HTP) methods for protein production will be an essential …

One of the first key steps in structural genomics is high-throughput expression and rapid
screening to select highly soluble proteins, the preferred candidates for crystal production …

A rapid unidirectional method for cloning PCR‐amplified cDNA fragments into virtually any
fusion protein expression vector is described. The method, termed PRESAT‐vector cloning …

Traditionally, the production of a recombinant protein requires a preliminary cloning step of
the target gene into an expression vector before evaluating its cellular expression. Among …

Production of soluble recombinant proteins is vital for structure–function analysis and
therapeutic applications. Unfortunately, when expressed in a heterologous host, such as …

Background The solubility of recombinant proteins expressed in bacteria is often
disappointingly low. Several strategies have been developed to improve the yield and one …

Gene fusion technology is a key tool in facilitating gene function studies. Hybrid molecules in
which all the components are joined precisely, without the presence of intervening and …

A suite of protein fusion vectors is presented that has been designed so that nine separate
fusion vectors can be constructed from one PCR product using InFusion™ cloning. These …