Background The self-sufficient autotransporter (AT) pathway, ubiquitous in Gram-negative
bacteria, combines a relatively simple protein secretion mechanism with a high transport …

Abstract Background The Autotransporter pathway, ubiquitous in Gram-negative bacteria,
allows the efficient secretion of large passenger proteins via a relatively simple mechanism …

Escherichia coli is still a very popular host for the production of recombinant proteins at an
analytical or industrial scale. Secretion of the proteins into the culture medium or display at …

Autotransporters (ATs) of Gram-negative bacteria contain an N-proximal passenger domain
that is transported to the extracellular milieu and a C-terminal β-domain that inserts into the …

The classical monomeric autotransporters are ubiquitously used by Gram-negative bacteria
to export virulence and colonization factors to their cell surface or into their surroundings …

Autotransporters (ATs) are large virulence factors secreted by Gram-negative bacteria. The
passenger domain, carrying the virulence functions, is transported across the bacterial outer …

Monomeric autotransporters have been extensively used for export of recombinant proteins
to the cell surface of Gram-negative bacteria. A bottleneck in the biosynthesis of such …

Background Cell display technologies (eg bacterial display) are attractive in directed
evolution as they provide the option to use flow-cytometric cell sorting for selection from …

Many virulence factors secreted by pathogenic Gram‐negative bacteria belong to the
autotransporter (AT) family. ATs consist of a passenger domain, which is the actual secreted …

Cell surface display of proteins can be used for several biotechnological applications such
as the screening of protein libraries, whole cell biocatalysis and live vaccine development …