Using microfluidics chips for live imaging and study of injury responses in Drosophila larvae

B Mishra, M Ghannad-Rezaie, J Li, X Wang… - JoVE (Journal of …, 2014 - jove.com
Live imaging is an important technique for studying cell biological processes, however this
can be challenging in live animals. The translucent cuticle of the Drosophila larva makes it …

[HTML][HTML] Microfluidic Chips for In Vivo Imaging of Cellular Responses to Neural Injury in Drosophila Larvae

M Ghannad-Rezaie, X Wang, B Mishra, C Collins… - PloS one, 2012 - journals.plos.org
With powerful genetics and a translucent cuticle, the Drosophila larva is an ideal model
system for live imaging studies of neuronal cell biology and function. Here, we present an …

Characterization of microfluidic clamps for immobilizing and imaging of Drosophila melanogaster larva's central nervous system

R Ghaemi, P Rezai, FR Nejad, PR Selvaganapathy - Biomicrofluidics, 2017 - pubs.aip.org
Drosophila melanogaster is a well-established model organism to understand biological
processes and study human diseases at the molecular-genetic level. The central nervous …

LarvaSPA, a method for mounting drosophila larva for long-term time-lapse imaging

H Ji, C Han - JoVE (Journal of Visualized Experiments), 2020 - jove.com
Live imaging is a valuable approach for investigating cell biology questions. The Drosophila
larva is particularly suited for in vivo live imaging because the larval body wall and most …

Immobilizing second-instar Drosophila larvae for imaging and surgery using the larva chip

LJ Smithson, TJ Waller… - Cold Spring Harbor …, 2024 - cshprotocols.cshlp.org
The simple body plan and semitranslucent cuticle of the Drosophila larva allow for imaging
of structures close to the body wall within intact animals. These include sensory neurons …

In vivo imaging of intact Drosophila larvae at sub-cellular resolution

Y Zhang, P Füger, SB Hannan, JV Kern… - JoVE (Journal of …, 2010 - jove.com
Recent improvements in optical imaging, genetically encoded fluorophores and genetic
tools allowing efficient establishment of desired transgenic animal lines have enabled …

On chip cryo-anesthesia of Drosophila larvae for high resolution in vivo imaging applications

AR Chaudhury, R Insolera, RD Hwang, YW Fridell… - Lab on a Chip, 2017 - pubs.rsc.org
We present a microfluidic chip for immobilizing Drosophila melanogaster larvae for high
resolution in vivo imaging. The chip creates a low-temperature micro-environment that …

Preparing individual Drosophila egg chambers for live imaging

TT Weil, RM Parton, I Davis - JoVE (Journal of Visualized Experiments), 2012 - jove.com
Live cell imaging is an important technique applied to a number of Drosophila tissues used
as models to investigate topics such as axis specification, cell differentiation and …

A Drosophila in vivo injury model for studying neuroregeneration in the peripheral and central nervous system

D Li, F Li, P Guttipatti, Y Song - JoVE (Journal of Visualized Experiments), 2018 - jove.com
The regrowth capacity of damaged neurons governs neuroregeneration and functional
recovery after nervous system trauma. Over the past few decades, various intrinsic and …

Simple microfluidic devices for in vivo imaging of C. elegans, Drosophila and zebrafish

S Mondal, S Ahlawat, SP Koushika - JoVE (Journal of Visualized …, 2012 - jove.com
Micro fabricated fluidic devices provide an accessible micro-environment for in vivo studies
on small organisms. Simple fabrication processes are available for microfluidic devices …