Characteristic Expression of Three Amylase-encoding Genes, agdA, amyB, and glaA in Aspergillus oryzae Transformants Containing Multiple Copies of the agdA …

T Minetoki, K Gomi, K Kitamoto… - Bioscience …, 1995 - academic.oup.com
In Aspergillus oryzae wild-type strains, the expression of the agdA gene encoding α-
glucosidase (AGL) is induced by maltose at the transcriptional level in a similar manner to …

Improvement of promoter activity by the introduction of multiple copies of the conserved region III sequence, involved in the efficient expression of Aspergillus oryzae …

T Minetoki, C Kumagai, K Gomi, K Kitamoto… - Applied microbiology …, 1998 - Springer
The role of the conserved sequence region III in the promoter regions of the amylase-
encoding genes amyB, glaA and agdA of Aspergillus oryzae was examined. Introduction of …

Nucleotide Sequence and Expression of α-Glucosidase-encoding Gene (agdA) from Aspergillus oryzae

T Minetoki, K Gomi, K Kitamoto… - Bioscience …, 1995 - academic.oup.com
We have isolated an α-glucosidase (AGL)-encoding gene (agdA) from Aspergillus oryzae by
heterologous hybridization using the corresponding Aspergillus niger gene as a probe …

Molecular Cloning and Characterization of a Transcriptional Activator Gene, amyR, Involved in the Amylolytic Gene Expression in Aspergillus oryzae

K Gomi, T Akeno, T Minetoki, K Ozeki… - Bioscience …, 2000 - academic.oup.com
A gene, designated amyR, coding for a transcriptional activator involved in amylolytic gene
expression has been cloned from Aspergillus oryzae by screening for a clone that enabled …

Insertion analysis of putative functional elements in the promoter region of the Aspergillus oryzae Taka-amylase A gene (amyB) using a heterologous Aspergillus …

Y Kanemori, K Gomi, K Kitamoto… - Bioscience …, 1999 - jstage.jst.go.jp
抄録 Expression of the Taka-amylase A gene (amyB) of Aspergillus oryzae is induced by
starch or maltose. The A. oryzae amyB gene promoter contains three highly conserved …

Deletion analysis of promoter elements of the Aspergillus oryzae agdA gene encoding α-glucosidase

T Minetoki, Y Nunokawa, K Gomi, K Kitamoto… - Current genetics, 1996 - Springer
The nucleotide sequence of a 1.5-kb fragment of the promoter region of the Aspergillus
oryzae agdA gene encoding α-glucosidase was determined. A comparison with the …

Functional elements of the promoter region of the Aspergillus oryzae glaA gene encoding glucoamylase

Y Hata, K Kitamoto, K Gomi, C Kumagai, G Tamura - Current genetics, 1992 - Springer
Analysis was made of the promoter region of the Aspergillus oryzae glaA gene encoding
glucoamylase. Northern blots using a glucoamylase cDNA as a probe indicated that the …

Construction of a fusion gene comprising the Taka-amylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus …

S Tada, K Gomi, K Kitamoto, K Takahashi… - Molecular and General …, 1991 - Springer
Northern blot analysis of glucose-grown and starch-grown mycelia of Aspergillus oryzae
R11340 was conducted using the cloned Taka-amylase A (TAA) gene as a probe. The …

Contribution Ratios of amyA, amyB, amyC Genes to High-Level α-Amylase Expression in Aspergillus oryzae

T Nemoto, J Maruyama… - Bioscience, biotechnology …, 2012 - academic.oup.com
Aspergillus oryzae strains express α-amylases abundantly, and the genome reference strain
RIB40 has three α-amylase genes (amyA, amyB, and amyC). However, there is no …

Distinct mechanism of activation of two transcription factors, AmyR and MalR, involved in amylolytic enzyme production in Aspergillus oryzae

K Suzuki, M Tanaka, Y Konno, T Ichikawa… - Applied microbiology …, 2015 - Springer
The production of amylolytic enzymes in Aspergillus oryzae is induced in the presence of
starch or maltose, and two Zn 2 Cys 6-type transcription factors, AmyR and MalR, are …