The TOL plasmid pWW53 encodes a catabolic pathway for the metabolism of toluene. It
bears an upper-pathway operon for the oxidation of toluene to benzoate and a copy of the …

Pseudomonas putida TOL plasmid pWW0 catabolic genes are clustered into two operons.
The first, the upper operon, is controlled by the xylR regulatory gene, whereas the second …

A simplified procedure to construct recombinant Pseudomonas putida (Pp) and related
bacteria, which transcribe conditionally specific genes inserted into their chromosome in …

Transcriptional lacZ fusions to the Pu and Pm promoters of the TOL (toluene degradation)
plasmid inserted in monocopy in the chromosome of Pseudomonas putida showed a very …

The XylR protein controls expression from the Pseudomonas putida TOL plasmid upper
pathway operon promoter (Pu) in response to aromatic effectors. XylR-dependent …

The activity of the ς 54-promoter Pu of Pseudomonas putida was examined in vitro with a
DNA template lacking upstream activating sequences, such that RNA polymerase can be …

The sequence elements determining the binding of the σ 54-containing RNA polymerase (σ
54-RNAP) to the Pu promoter of Pseudomonas putida have been examined. Contrary to …

The effect of FtsH, an essential inner membrane‐bound protease, in the regulation of the
σ54‐dependent Pu promoter has been examined in vivo. Escherichia coli cells lacking FtsH …

▪ Abstract The xyl genes of Pseudomonas putida TOL plasmid that specify catabolism of
toluene and xylenes are organized in four transcriptional units: the upper-operon …

The gene cluster adjacent to the sequence of rpoN (encoding sigma factor ς 54)
ofPseudomonas putida has been studied with respect to the C source regulation of the Pu …