Activation of the Pseudomonas TOL plasmid upper pathway operon. Identification of binding sites for the positive regulator XylR and for integration host factor protein

MA Abril, M Buck, JL Ramos - Journal of Biological Chemistry, 1991 - ASBMB
Expression of the Pseudomonas putida TOL plasmid upper pathway operon requires a
promoter that belongs to the-12/-24 class. Stimulation of transcription from this promoter is …

Promoter‐upstream activator sequences are required for expression of the xylS gene and upper‐pathway operon on the Pseudomonas TOL plasmid

A Holtel, MA Abril, S Marques, KN Timmis… - Molecular …, 1990 - Wiley Online Library
Stimulation of transcription from the Pseudomonas TOL plasmid xylS gene promoter (Ps)
and the upperpathway operon promoter (Pu) is dependent on the positive regulator protein …

Upstream binding sequences of the XyIR activator protein and integration host factor in the xylS gene promoter region of the Pseudomonas TOL plasmid

A Holtel, KN Timmis, JL Ramos - Nucleic acids research, 1992 - academic.oup.com
The xylR and xylS genes, which encode the positive regulators of the TOL plasmid catabolic
pathways, are adjacent genes on the TOL plasmid and are transcribed from divergent …

Physical and Functional Analysis of the Prokaryotic Enhancer of the σ54-promoters of the TOL Plasmid ofPseudomonas putida

J Pérez-Martı́n, V de Lorenzo - Journal of molecular biology, 1996 - Elsevier
The physical and the functional organization of the upstreamcis-acting sequence that
controls at a distance the transcriptional activity ofPuandPs, the two σ54-dependent …

TRANSCRIPTIONAL CONTROL OF THE PSEUDOMONAS TOL PLASMID CATABOLIC OPERONS IS ACHIEVED THROUGH AN INTERPLAY OF HOST FACTORS …

JL Ramos, S Marqués, KN Timmis - Annual review of …, 1997 - annualreviews.org
▪ Abstract The xyl genes of Pseudomonas putida TOL plasmid that specify catabolism of
toluene and xylenes are organized in four transcriptional units: the upper-operon …

Activation and Repression of Transcription at the Double Tandem Divergent Promoters for the xylR and xylS Genes of the TOL Plasmid of Pseudomonas putida

S Marqués, MT Gallegos, M Manzanera… - Journal of …, 1998 - Am Soc Microbiol
The xylR and xylS genes are divergent and control transcription of the TOL plasmid
catabolic pathways for toluene metabolism. Four promoters are found in the 300-bp …

The sigma 54-dependent promoter Ps of the TOL plasmid of Pseudomonas putida requires HU for transcriptional activation in vivo by XylR

J Pérez-Martín, V de Lorenzo - Journal of bacteriology, 1995 - Am Soc Microbiol
In the presence of toluene and xylenes, the sigma 54-dependent Ps promoter of the TOL
(toluene biodegradation) plasmid pWW0 of Pseudomonas putida is activated at a distance …

Functional domains of the TOL plasmid transcription factor XylS

N Kaldalu, U Toots, V de Lorenzo… - Journal of bacteriology, 2000 - Am Soc Microbiol
The alkylbenzoate degradation genes of Pseudomonas putida TOL plasmid are positively
regulated by XylS, an AraC family protein, in a benzoate-dependent manner. In this study …

Signal-regulator interactions, genetic analysis of the effector binding site of xyls, the benzoate-activated positive regulator of Pseudomonas TOL plasmid meta …

JL Ramos, C Michan, F Rojo, D Dwyer… - Journal of molecular …, 1990 - Elsevier
This study reports a genetic analysis of the interactions between a positive regulator of gene
expression and its effector molecules. Transcription of the TOL plasmid meta-cleavage …

In vitro activities of an N-terminal truncated form of XylR, a σ54-dependent transcriptional activator of Pseudomonas putida

J Pérez-Martı́n, V de Lorenzo - Journal of molecular biology, 1996 - Elsevier
A truncated derivative of the XylR protein, which is able to constitutively activate the σ54-
dependentPupromoter of the TOL (toluene biodegradation) plasmid ofPseudomonas putida …