The effects of fat harvesting and preparation, air exposure, obesity, and stem cell enrichment on adipocyte viability prior to graft transplantation

R Cucchiani, L Corrales - Aesthetic Surgery Journal, 2016 - academic.oup.com
Background Adipocyte viability is affected by fat preparation and processing methods, but
rigorous and objective studies of these relationships are lacking. Objectives The authors …

Improving fat transfer viability: the role of aging, body mass index, and harvest site

PJ Geissler, K Davis, J Roostaeian… - Plastic and …, 2014 - journals.lww.com
Background: Fat grafting remains unpredictable in the clinical setting, and variables that
influence adipocyte survival, such as age, body mass index, and specific donor sites, are still …

Human adipocyte viability testing: a new assay

JW MacRae, SS Tholpady, AJ Katz… - Aesthetic Surgery …, 2003 - academic.oup.com
Background Surgical experience and anecdotal data on the most effective method of
harvesting, preparing, and injecting autologous fat grafts are inconsistent and conflicting …

Wet and dry techniques for structural fat graft harvesting: histomorphometric and cell viability assessments of lipoaspirated samples

T Agostini, D Lazzeri, A Pini, G Marino… - Plastic and …, 2012 - journals.lww.com
Background: Autologous fat transplantation is among the surgical procedures performed
most commonly by plastic surgeons for cosmetic and/or reconstructive purposes. In the …

The importance of protecting the structure and viability of adipose tissue for fat grafting

Y He, X Zhang, X Han, F Li - Plastic and Reconstructive Surgery, 2022 - journals.lww.com
Background: Fat grafting is widely used for soft-tissue augmentation; however, the related
clinical outcome remains variable and technique-dependent. The mechanisms underlying …

Effects of centrifugation on cell composition and viability of aspirated adipose tissue processed for transplantation

A Condé-Green, LS Baptista… - Aesthetic Surgery …, 2010 - academic.oup.com
Background: Centrifugation is one of the preferred methods of fat processing. Although it has
been promoted for nearly three decades to separate adipose tissue components before …

Comparative analysis of processing methods in fat grafting

HM Salinas, GF Broelsch, JR Fernandes… - Plastic and …, 2014 - journals.lww.com
Background: Centrifugation is a popular processing method, with an unclear mechanism of
action. Hypotheses include fat concentration, reduced inflammatory response by removal of …

Blood impairs viability of fat grafts and adipose stem cells: importance of washing in fat processing

S Jin, Z Yang, X Han, F Li - Aesthetic Surgery Journal, 2021 - academic.oup.com
Background Fat processing plays a pivotal role in graft survival. Each component of the
blood in lipoaspirate affects fat survival in different ways, but the mechanisms are not clear …

Autologous fat grafting: a comparative study of four current commercial protocols

V Hivernaud, B Lefourn, M Robard, J Guicheux… - Journal of Plastic …, 2017 - Elsevier
Background Autologous fat grafting is a widely used technique that gives natural results
when treating soft tissue deficiencies. However, there is no consensus on which is the best …

The influence of low-and high-negative-pressure liposuction and different harvesting sites on the viability and yield of adipocytes and other nucleated cells

M Molitor, M Trávníčková, O Měšťák… - Aesthetic Plastic …, 2021 - Springer
Background The volume effect of fat grafting is highly dependent on the presence of viable
adipocytes and other nucleated cells within the lipoaspirate. We suspected that one of the …