PCR-based strategy to generate integrative targeting alleles with large regions of homology |
CiNii Research CiNii 国立情報学研究所 学術情報ナビゲータ[サイニィ] 詳細へ移動 検索フォームへ …

Multiple gene knockouts play an important role in metabolic engineering. The flanked
homology length, homologous to the region adjacent to the target gene, of the knockout …

Background The cloning of gene sequences forms the basis for many molecular biological
studies. One important step in the cloning process is the isolation of bacterial transformants …

The full length of interested genes can be usually cloned by assembling exons or RACE
products through overlap PCR. However, the procedure requires multiple PCR steps, which …

The ever increasing pace of genome sequence acquisition, annotation, and transcriptional
profiling is providing large data sets of possible open reading frame function. However …

Genetic engineering to enhance Page 1 G 1996 Nature Publishing Group http://www.nature.com/naturebiotechnology
YYCGG CCY YGS GG GGLGL GGLGGGG SGLLS SGG GLLYLYLLLY Genetic engineering …

The use of bacterial transposon mutant libraries in phenotypic screens is a well-established
technique for determining which genes are essential or advantageous for growth in …

Abstract Background The Gateway recombinatorial cloning system allows easy and rapid
joining of DNA fragments. Here we report the construction and evaluation of three different …

Genome sequencing of a large number of organisms has provided a wealth of previously
uncharacterized genes. Rapid functional analysis of these genes relies on efficient methods …

A PCR cloning method that combines a dual selection pGATE-1 plasmid vector and an
improved overlap extension cloning was developed. This efficient and cost-effective method …