Interspecific complementation of an Escherichia coli recA mutant was used to identify
recombinant plasmids within a genomic cosmid library derived from Neisseria gonorrhoeae …

The nucleotide sequence of the recA gene of Neisseria gonorrhoeae MS11 has been
determined. The product of this gene can act as a recombinase in Escherichia coli, but does …

The paradigm of homologous recombination comes from Escherichia coli, where the genes
involved have been segregated into pathways. In the human pathogen Neisseria …

In the pathogen Neisseria gonorrhoeae (Gc), the RecA protein is necessary for DNA repair,
DNA transformation and pilus antigenic variation. Many bacteria contain a gene, recX, which …

Two classes of recA mutations have been constructed for use in Neisseria gonorrhoeae:
three insertionally inactivated (knockout') mutations and three LacI-regulatable constructs …

We previously identified and genetically characterized several factors essential for the
natural competence of transformation in Neisseria gonorrhoeae. Here we analyse the …

Escherichia coli RecX (RecX Ec) is a negative regulator of RecA activities both in the
bacterial cell and in vitro. In contrast, the Neisseria gonorrhoeae RecX protein (RecX Ng) …

Most strains of Neisseria gonorrhoeae carry the 57-kb gonococcal genetic island (GGI), as
do a few strains of Neisseria meningitidis. The GGI is inserted into the chromosome at the dif …

An origin of replication (ori) was obtained from a naturally occurring β-lactamase-producing
plasmid isolated from Neisseria gonorrhoeae and used to construct shuttle vectors capable …

We created plasmids for use in insertion-duplication mutagenesis (IDM) of Neisseria
gonorrhoeae. This mutagenesis method has the advantage that it requires only a single …