Engineering Trichoderma reesei for Hyperproduction of Cellulases on Glucose to Efficiently Saccharify Pretreated Corncobs

F Zheng, R Yang, Y Cao, W Zhang, X Lv… - Journal of Agricultural …, 2020 - ACS Publications
The filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina) is widely used
as a cellulase producer in the industry. Herein, we describe the rational engineering of the …

Reformulating the Hydrolytic Enzyme Cocktail of Trichoderma reesei by Combining XYR1 Overexpression and Elimination of Four Major Cellulases to Improve …

W Zhang, J Guo, X Wu, Y Ren, C Li… - Journal of Agricultural …, 2021 - ACS Publications
The industrial fungus Trichoderma reesei has an outstanding capability of secreting an
enzyme cocktail comprising multiple plant biomass-degrading enzymes. Herein, the …

Efficient preparation of soluble inducer for cellulase production and saccharification of corn stover using in-house generated crude enzymes

Y Li, P Zhang, D Zhu, B Yao, T Hasunuma… - Biochemical …, 2022 - Elsevier
Although high-level cellulase production by Trichoderma reesei from lignocellulosic
biomass in biorefinery has been widely studied, more efficient and economic hydrolysis is …

Bioprocessing of enhanced cellulase production from a mutant of Trichoderma asperellum RCK2011 and its application in hydrolysis of cellulose

S Raghuwanshi, D Deswal, M Karp, RC Kuhad - Fuel, 2014 - Elsevier
A mutant strain of Trichoderma asperellum RCK2011 was developed through UV-irradiation
for enhanced cellulase production and lower catabolite repression. The production of …

Production of highly efficient cellulase mixtures by genetically exploiting the potentials of Trichoderma reesei endogenous cellulases for hydrolysis of corncob …

Y Qian, L Zhong, J Gao, N Sun, Y Wang, G Sun… - Microbial Cell …, 2017 - Springer
Background Trichoderma reesei is one of the most important fungi utilized for cellulase
production. However, its cellulase system has been proven to be present in suboptimal ratio …

Inducer-free cellulase production system based on the constitutive expression of mutated XYR1 and ACE3 in the industrial fungus Trichodermareesei

T Arai, S Ichinose, N Shibata, H Kakeshita… - Scientific Reports, 2022 - nature.com
Trichoderma reesei is a widely used host for producing cellulase and hemicellulase
cocktails for lignocellulosic biomass degradation. Here, we report a genetic modification …

On-site cellulase production and efficient saccharification of corn stover employing cbh2 overexpressing Trichoderma reesei with novel induction system

Y Li, X Zhang, L Xiong, MA Mehmood, X Zhao… - Bioresource …, 2017 - Elsevier
Although on-site cellulase production offers cost-effective saccharification of lignocellulosic
biomass, low enzyme titer is still a barrier for achieving robustness. In the present study, a …

Production of the versatile cellulase for cellulose bioconversion and cellulase inducer synthesis by genetic improvement of Trichoderma reesei

J Gao, Y Qian, Y Wang, Y Qu, Y Zhong - Biotechnology for Biofuels, 2017 - Springer
Background The enzymes for efficient hydrolysis of lignocellulosic biomass are a major
factor in the development of an economically feasible cellulose bioconversion process. Up …

Efficient crude multi-enzyme produced by Trichoderma reesei using corncob for hydrolysis of lignocellulose

F Jiang, L Ma, R Cai, Q Ma, G Guo, L Du, D Xiao - 3 Biotech, 2017 - Springer
To improve the efficiency of enzymatic saccharification for lignocellulose, an efficient crude
multi-enzyme was produced by Trichoderma reesei using corncob, a low cost inducer …

Utilization of agricultural residues for enhancement of cellulolytic enzyme production and enzymatic saccharification by Trichoderma harzianum KUC1716

H Lee, YM Lee, YM Heo, J Lee, JS Kim… - Industrial Crops and …, 2017 - Elsevier
Agricultural residues are a potentially economical and eco-friendly source for second-
generation ethanol production. In the present study, efficient cellulolytic enzyme production …