Immobilizing second-instar Drosophila larvae for imaging and surgery using the larva chip

LJ Smithson, TJ Waller… - Cold Spring Harbor …, 2024 - cshprotocols.cshlp.org
The simple body plan and semitranslucent cuticle of the Drosophila larva allow for imaging
of structures close to the body wall within intact animals. These include sensory neurons …

Long-term in vivo imaging of Drosophila larvae

P Kakanj, SA Eming, L Partridge, M Leptin - Nature Protocols, 2020 - nature.com
The Drosophila larva has been used to investigate many processes in cell biology, including
morphogenesis, physiology and responses to drugs and new therapeutic compounds …

Using microfluidics chips for live imaging and study of injury responses in Drosophila larvae

B Mishra, M Ghannad-Rezaie, J Li, X Wang… - JoVE (Journal of …, 2014 - jove.com
Live imaging is an important technique for studying cell biological processes, however this
can be challenging in live animals. The translucent cuticle of the Drosophila larva makes it …

Microfluidic Chips for In Vivo Imaging of Cellular Responses to Neural Injury in Drosophila Larvae

M Ghannad-Rezaie, X Wang, B Mishra, C Collins… - PloS one, 2012 - journals.plos.org
With powerful genetics and a translucent cuticle, the Drosophila larva is an ideal model
system for live imaging studies of neuronal cell biology and function. Here, we present an …

[HTML][HTML] LarvaSPA, a method for mounting Drosophila larva for long-term time-lapse imaging

H Ji, C Han - Journal of visualized experiments: JoVE, 2020 - ncbi.nlm.nih.gov
Live imaging is a valuable approach for investigating cell biology questions. The Drosophila
larva is particularly suited for in vivo live imaging because the larval body wall and most …

In vivo imaging of intact Drosophila larvae at sub-cellular resolution

Y Zhang, P Füger, SB Hannan, JV Kern… - JoVE (Journal of …, 2010 - jove.com
Recent improvements in optical imaging, genetically encoded fluorophores and genetic
tools allowing efficient establishment of desired transgenic animal lines have enabled …

A solid-phase immunostaining protocol for high-resolution imaging of delicate structures in the Drosophila larval central nervous system (CNS)

EA Daubert, BG Condron - Cold Spring Harbor Protocols, 2007 - cshprotocols.cshlp.org
METHOD 1. Under a dissecting microscope, dissect the CNS from the larva in at least 3 ml of
1% PS insect medium at room temperature. Immediately transfer the CNS to 3 ml of clean …

On chip cryo-anesthesia of Drosophila larvae for high resolution in vivo imaging applications

AR Chaudhury, R Insolera, RD Hwang, YW Fridell… - Lab on a Chip, 2017 - pubs.rsc.org
We present a microfluidic chip for immobilizing Drosophila melanogaster larvae for high
resolution in vivo imaging. The chip creates a low-temperature micro-environment that …

Collection and mounting of Drosophila embryos for imaging

RM Parton, AM Vallés, IM Dobbie… - Cold Spring Harbor …, 2010 - cshprotocols.cshlp.org
CSH Protocols_Inside STYLE FILE Page 1 Collection and Mounting of Drosophila Embryos for
Imaging Richard M. Parton, Ana Maria Vallés, Ian M. Dobbie, and Ilan Davis INTRODUCTION …

Dissection and immunolabeling of the central and peripheral nervous system of Drosophila larvae

K Clayworth, M Gilbert, V Auld - Cold Spring Harbor …, 2024 - cshprotocols.cshlp.org
The ability to visualize the cells and proteins of a tissue within their original context (ie, in
vivo) is invaluable for the study of that biological system. Visualization is especially important …