Immobilizing second-instar Drosophila larvae for imaging and surgery using the larva chip
LJ Smithson, TJ Waller… - Cold Spring Harbor …, 2024 - cshprotocols.cshlp.org
The simple body plan and semitranslucent cuticle of the Drosophila larva allow for imaging
of structures close to the body wall within intact animals. These include sensory neurons …
of structures close to the body wall within intact animals. These include sensory neurons …
Long-term in vivo imaging of Drosophila larvae
P Kakanj, SA Eming, L Partridge, M Leptin - Nature Protocols, 2020 - nature.com
The Drosophila larva has been used to investigate many processes in cell biology, including
morphogenesis, physiology and responses to drugs and new therapeutic compounds …
morphogenesis, physiology and responses to drugs and new therapeutic compounds …
Using microfluidics chips for live imaging and study of injury responses in Drosophila larvae
Live imaging is an important technique for studying cell biological processes, however this
can be challenging in live animals. The translucent cuticle of the Drosophila larva makes it …
can be challenging in live animals. The translucent cuticle of the Drosophila larva makes it …
Microfluidic Chips for In Vivo Imaging of Cellular Responses to Neural Injury in Drosophila Larvae
With powerful genetics and a translucent cuticle, the Drosophila larva is an ideal model
system for live imaging studies of neuronal cell biology and function. Here, we present an …
system for live imaging studies of neuronal cell biology and function. Here, we present an …
[HTML][HTML] LarvaSPA, a method for mounting Drosophila larva for long-term time-lapse imaging
H Ji, C Han - Journal of visualized experiments: JoVE, 2020 - ncbi.nlm.nih.gov
Live imaging is a valuable approach for investigating cell biology questions. The Drosophila
larva is particularly suited for in vivo live imaging because the larval body wall and most …
larva is particularly suited for in vivo live imaging because the larval body wall and most …
In vivo imaging of intact Drosophila larvae at sub-cellular resolution
Y Zhang, P Füger, SB Hannan, JV Kern… - JoVE (Journal of …, 2010 - jove.com
Recent improvements in optical imaging, genetically encoded fluorophores and genetic
tools allowing efficient establishment of desired transgenic animal lines have enabled …
tools allowing efficient establishment of desired transgenic animal lines have enabled …
A solid-phase immunostaining protocol for high-resolution imaging of delicate structures in the Drosophila larval central nervous system (CNS)
EA Daubert, BG Condron - Cold Spring Harbor Protocols, 2007 - cshprotocols.cshlp.org
METHOD 1. Under a dissecting microscope, dissect the CNS from the larva in at least 3 ml of
1% PS insect medium at room temperature. Immediately transfer the CNS to 3 ml of clean …
1% PS insect medium at room temperature. Immediately transfer the CNS to 3 ml of clean …
On chip cryo-anesthesia of Drosophila larvae for high resolution in vivo imaging applications
AR Chaudhury, R Insolera, RD Hwang, YW Fridell… - Lab on a Chip, 2017 - pubs.rsc.org
We present a microfluidic chip for immobilizing Drosophila melanogaster larvae for high
resolution in vivo imaging. The chip creates a low-temperature micro-environment that …
resolution in vivo imaging. The chip creates a low-temperature micro-environment that …
Collection and mounting of Drosophila embryos for imaging
RM Parton, AM Vallés, IM Dobbie… - Cold Spring Harbor …, 2010 - cshprotocols.cshlp.org
CSH Protocols_Inside STYLE FILE Page 1 Collection and Mounting of Drosophila Embryos for
Imaging Richard M. Parton, Ana Maria Vallés, Ian M. Dobbie, and Ilan Davis INTRODUCTION …
Imaging Richard M. Parton, Ana Maria Vallés, Ian M. Dobbie, and Ilan Davis INTRODUCTION …
Dissection and immunolabeling of the central and peripheral nervous system of Drosophila larvae
K Clayworth, M Gilbert, V Auld - Cold Spring Harbor …, 2024 - cshprotocols.cshlp.org
The ability to visualize the cells and proteins of a tissue within their original context (ie, in
vivo) is invaluable for the study of that biological system. Visualization is especially important …
vivo) is invaluable for the study of that biological system. Visualization is especially important …
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