Electrophoretically uniform fluorescent dyes for automated DNA sequencing
ML Metzker, J Lu, RA Gibbs - Science, 1996 - science.org
A class of dyes, BODIPY fluorophores, has been identified for automated DNA sequencing
that has improved spectral characteristics compared with conventional fluorescein and …
that has improved spectral characteristics compared with conventional fluorescein and …
Alternative dye-labeled primers for automated DNA sequencing
ML Metzker, RA Gibbs - US Patent 5,861,287, 1999 - Google Patents
Methods for the use of a class of dyes for improved DNA sequencing are provided. A new
class of dyes, BODIPY® fluorophores, has been described recently. The parent heterocyclic …
class of dyes, BODIPY® fluorophores, has been described recently. The parent heterocyclic …
Alternative dye-labeled ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated DNA analysis
ML Metzker, RA Gibbs - US Patent 5,728,529, 1998 - Google Patents
Methods for the use of a class of dyes for improved DNA sequencing by the chain
termination method of DNA sequencing, and internal labelling of polynucleotides by …
termination method of DNA sequencing, and internal labelling of polynucleotides by …
A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides
JM Prober, GL Trainor, RJ Dam, FW Hobbs… - Science, 1987 - science.org
A DNA sequencing system based on the use of a novel set of four chain-terminating
dideoxynucleotides, each carrying a different chemically tuned succinylfluorescein dye …
dideoxynucleotides, each carrying a different chemically tuned succinylfluorescein dye …
Alternative dye-labeled primers for automated DNA sequencing
ML Metzker, RA Gibbs - US Patent 5,614,386, 1997 - Google Patents
Methods for the use of a class of dyes for improved DNA sequencing are provided. A new
class of dyes, BODIPYG) fluorophore, has been described recently. The parent hetero cyclic …
class of dyes, BODIPYG) fluorophore, has been described recently. The parent hetero cyclic …
The future of DNA sequencing
LM Smith - Science, 1993 - science.org
Lloyd M. Smith cross-linked solutions are liquid, albeit vis-cous, and thus may in principle be
pumped in and out of theelectrophoresis cell as desired. They offer for the first time the possi …
pumped in and out of theelectrophoresis cell as desired. They offer for the first time the possi …
One-tube post-PCR fluorescent labeling of DNA fragments.
M Inazuka, T Tahira, K Hayashi - Genome research, 1996 - genome.cshlp.org
A method for fluorescent postlabeling of PCR products has been developed. The method
uses Klenow fragment of DNA polymerase I that exchanges the 3'-terminal residue of PCR …
uses Klenow fragment of DNA polymerase I that exchanges the 3'-terminal residue of PCR …
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
TS Seo, X Bai, DH Kim, Q Meng, S Shi… - Proceedings of the …, 2005 - National Acad Sciences
We report four-color DNA sequencing by synthesis (SBS) on a chip, using four
photocleavable fluorescent nucleotide analogues (dGTP-PC-Bodipy-FL-510, dUTP-PC …
photocleavable fluorescent nucleotide analogues (dGTP-PC-Bodipy-FL-510, dUTP-PC …
Optimization of parameters in a DNA sequenator using fluorescence detection
H Kambara, T Nishikawa, Y Katayama, T Yamaguchi - Bio/Technology, 1988 - nature.com
We have developed an automated DNA sequenator using a real-time fluorescence
detection method, and achieved excellent sensitivity and rapid analysis by optimizing …
detection method, and achieved excellent sensitivity and rapid analysis by optimizing …
Multiplex DNA sequencing
GM Church, S Kieffer-Higgins - Science, 1988 - science.org
The increasing demand for DNA sequences can be met by replacement of each DNA
sample in a device with a mixture of N samples so that the normal throughput is increased …
sample in a device with a mixture of N samples so that the normal throughput is increased …