There are growing numbers of recombinant proteins that have been expressed in milk. Thus
one can consider the placement of any gene of interest under the control of the regulatory …

Recombinant coagulation factor IX must be produced in mammalian cells because FIX
synthesis involves translational modifications. Human cell culture-based expression of …

Production of biopharmaceuticals from transgenic animal milk is a cost‐effective method for
highly complex proteins that cannot be efficiently produced using conventional systems such …

Transgenic livestock may prove useful for the large scale production of valuable proteins. By
targeting expression to the mammary gland these proteins could be harvested from milk. To …

Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene
(neo) and a human coagulation factor IX genomic construct designed for expression of the …

To improve the available values of transgenic animals, we produced a mutant human
coagulation factor IX minigene (including cDNA and intron I) with arginine at 338 changed to …

Human factor IX is synthesized in the liver and secreted in the blood, where it participates in
a group of reactions involving coagulation factors and proteins that permit sanguinary …

Gene transfer into animals has considerable potential for livestock improvement. If this
potential is to be realized, the ease of generation of transgenic livestock will be of major …

1 Materials and methods 1.1 Materials All kinds of restriction enzymes and ligase were
purchased from New England Biolabs, the USA. The retrovirus packaging cell line PA317 …

The efficiency of nuclear transfer (NT) using two primary cultures of fetal fibroblasts (FF1 and
FF2) was compared vs. the same cultures transfected with an expression vector in which the …