The past year has seen the publication of a number of papers describing the identification of
cytosolic factors involved in import of proteins to the nucleus. Although, at first glance, this …

To enter the nucleus, a protein must transit through one of the largest and most complex
gateways in the eukary-otic cell, the nuclear pore. Consisting of perhaps 1000 proteins (60 …

The past two years have seen a significant increase in our understanding of nuclear protein
import. Five cytosolic import factors have been identified, two of which have been shown to …

Transport of proteins, RNAs and ribonucleoprotein particles into and out of the nucleus is
essential for many cellular functions to proceed. Recent progress in this area of research …

Publisher Summary The availability of the green fluorescent protein (GFP) from Aequorea
victoria has revolutionized in vivo studies of dynamic processes. A simple combination of …

The term nuclear transport, refers to the movement of a large variety of macromolecules both
into and out of the nucleus. Transport must be extremely selective, yet also very efficient. A …

After synthesis in the cytoplasm, nuclear proteins traverse the nuclear envelope as a result
of the specific recognition of nuclear localization signals by import. Various approaches …

Nuclear proteins are actively and posttranslationally transported across the nuclear
envelope. This transport is highly selective process that can be divided into two steps …

We have developed an in vitro system involving digitonin-permeabilized vertebrate cells to
study biochemical events in the transport of macromolecules across the nuclear envelope …

Large nuclear proteins must possess a signal sequence to pass through the nuclear pores.
Using an in vitro system, we have been able experimentally to dissect nuclear protein …