Due to the difficulty of multiple deletions using the Cre/loxP system, a simple, markerless
multiple-deletion method based on a Cre/mutant lox system combining a right-element (RE) …

Genome rearrangement is an increasingly important technique to facilitate the
understanding of genome functions. A Cre/loxP-mediated deletion system for large-scale …

A precise and scarless genome excision method, employing the Cre/loxP system in concert
with double-strand break (DSB)-stimulated intramolecular recombination was developed …

A simple and random genome deletion method combining insertion sequence (IS) element
IS 31831 and the Cre/loxP excision system generated 42 Corynebacterium glutamicum …

ABSTRACT A simple and high-throughput transposon-mediated mutagenesis system
employing two different types of transposons in combination with direct genomic DNA …

The engineering of Corynebacterium glutamicum is important for enhanced production of
biochemicals. To construct an improved C. glutamicum genome, we developed a precise …

Owing to the increasing demand for amino acids and valuable commodities that can be
produced by Corynebacterium glutamicum, there is a pressing need for new rapid genome …

Gene deletion techniques are important for modifying Corynebacterium glutamicum, the
bacterium for industrial production of amino acids. In this study, a novel multiple-gene …

The engineering of Corynebacterium glutamicum is important for enhanced production of
biochemicals. To construct an optimal C. glutamicum genome, a precise site-directed gene …

Corynebacterium glutamicum has played a principal role in the progress of the amino acid
fermentation industry. The complete genome sequence of the representative wild-type strain …