Recent advances in the cryopreservation of mouse sperm have resulted in dramatically
improved in vitro fertilization (IVF) rates, but the biological mechanisms underlying the …

Sperm cryopreservation is useful for the effective storage of genomic resources derived from
genetically engineered mice. However, freezing the sperm of C57BL/6 mice, the most …

Background In vitro fertilization (IVF) of eggs by frozen and thawed C57BL/6J mouse sperm
is inhibited by dead sperm and enhanced by preincubation of the sperm in calcium-free …

Various antioxidant strategies such as supplementation of antioxidants, limiting oxygen
concentration with Oxyrase, and reducing reactive oxygen species through mild …

Although cryopreservation of ejaculated sperm has been in clinical and agricultural use for
decades, it is not completely clear how the damage that sperm incur as a result of …

Currently the most popular mouse sperm freezing medium is R18SM3+ MTG containing
18% raffinose, 3% skim milk, and 0.5 mM monothioglycerol (MTG), but there is no …

Mouse sperm cryopreservation has greatly developed and spread throughout the world
since it was the first successfully performed in 1990. The technology of sperm …

Osmotic stress caused oxidative stress in rhesus macaque sperm, which was alleviated by
antioxidant supplementation. The objective of the present study was to demonstrate that …

Sperm become most sensitive to cold shock when cooled from 37° C to 5° C at rates that are
too fast or too slow; cold shock increases the susceptibility to oxidative damage owing to its …

Assisted reproduction using frozen‐thawed semen has practical advantages, although
cryopreservation is detrimental to sperm fertility in most mammals. We examined the …