Site-directed mutagenesis is still a very efficient strategy to elaborate improved enzymes.
Recently, advances have been made in developing rational strategies aimed at reshaping …

The challenging field of de novo enzyme design is beginning to produce exciting results.
The application of powerful computational methods to functional protein design has recently …

Computational enzyme design has emerged as a promising tool for generating made-to-
order biocatalysts. In addition to improving the reliability of the design cycle, current efforts in …

Current computational tools to assist experimentalists for the design and engineering of
proteins with desired catalytic properties are reviewed. The applications of these tools for de …

Protein engineering techniques have been available for over two decades beginning with
the development of methods for genetic engineering. Since that time, the engineering of …

Enzyme engineering is undergoing the most profound and exciting transformation in its
history 1. It promises unprecedented expansion in the scope and applications of modified or …

The de novo design of enzymes with activities not found in natural biocatalysts is a major
challenge for molecular biology. Sophisticated computational methods have recently led to …

Many research groups successfully rely on whole-gene random mutagenesis and
recombination approaches for the directed evolution of enzymes. Recent advances in …

Highlights•The most recent developments of de novo enzyme design (methodologies and
applications) are reported.•The different chemical reactions for which de novo enzymes …

The ability of one enzyme to catalyse multiple, mechanistically distinct transformations likely
played a crucial role in organisms' abilities to adapt to changing external stimuli in the past …