Cytoplasmic maturation as determined by male pronuclear formation following fertilization in
vitro was examined in pig oocytes cultured under different hormonal conditions during either …

An intense interest exists internationally in investigating in vitro procedures for the
maturation, fertilization, and culture of immature oocytes from domestic mammalian species …

Abstract Thimerosal (200 gAM) triggered Ca2+ oscillations in 56 of 56 mature porcine
oocytes. The Ca2+ oscillations were blocked by the sulfhydryl-reducing agent dithiothreitol …

Fresh horse oocytes dissected from slaughterhouse ovaries were allocated to two groups
depending on the degree of development of the cumulus investment. In Group I, the cumulus …

Various procedures have been reported for successful in vitro maturation and in vitro
fertilization (IVM/IVF) of bovine follicular oocytes. Direct comparisons of these different …

Abstract! The objective of this study was to perform intracytoplasmic sperm injection (ICSI)
on in vitro matured equine oocytes and to improve in vitro embryonic development on Vero …

In 33 trials, 435 cattle oocytes were matured in vitro in modified Krebs-Ringer solution. The
fertilization rate using spermatozoa capacitated in a chemically defined medium ranged from …

Since the birth of the first calf from an in-vitro fertilized oocyte (Brackett et al., 1982), in-vitro
fertilization has been achieved in two other ruminant species: sheep and goats. Efficient in …

In vitro matured pig oocytes were activated using a combined treatment of calcium
ionophore A 23187 with cycloheximide. The oocytes were exposed to ionophore (10, 25 or …

In vitro embryo production is not yet successful in the horse, largely due to low rates of
fertilization in vitro. However, methods to produce embryos from isolated oocytes have been …