Oocyte maturation, sperm capacitation and pre-implantation development in the bovine: implications for in vitro production of embryos.

A Soom, A Kruif - 1996 - cabidigitallibrary.org
This review discusses the state of the art in respect of the in vitro production of cattle
embryos. It is suggested that embryo production and quality might be improved by systems …

[PDF][PDF] Activation of cumulus-free equine oocytes: effect of maturation medium, calcium ionophore concentration and duration of cycloheximide exposure

YH Choi, CC Love, DD Varner… - REPRODUCTION …, 2001 - researchgate.net
Offspring cloned from somatic cells have been reported in several species, including sheep
(Wilmut et al., 1997), mice (Wakayama et al., 1998), cattle (Kato et al., 1998), goats (Baguisi …

Functional analysis of activation of porcine oocytes by spermatozoa, calcium ionophore, and electrical pulse

WH Wang, LR Abeydeera, RS Prather… - Molecular …, 1998 - Wiley Online Library
The present study was conducted to examine differences in the activation of pig oocytes
induced by sperm penetration or the artificial stimulators calcium ionophore A23187 and …

Equine embryos at the compacted morula and blastocyst stage can be obtained by intracytoplasmic sperm injection (ICSI) of in vitro matured oocytes with frozen …

G Lazzari, G Crotti, P Turini, R Duchi, G Mari… - 2002 - cabidigitallibrary.org
The objectives of this study were to:(1) investigate the efficiency of the intracytoplasmic
sperm injection (ICSI) procedure by using in vitro matured oocytes and frozen-thawed …

[PDF][PDF] In vitro fertilization and embryo development in pigs

LR Abeydeera - REPRODUCTION-CAMBRIDGE …, 2001 - biosciproceedings.org
Considerable progress has been made in the in vitro production of pig embryos using
improved methods for in vitro maturation(IVM) and fertilization(IVF). Despite the progress …

Bovine oocyte maturation, fertilization and further development in vitro and after transfer into recipients

MI Prokofiev, LK Ernst, NM Suraeva, IS Lagutina… - Theriogenology, 1992 - Elsevier
Bovine oocytes were aspirated from ovaries within 1.6 to 2 hours after slaughter. They were
then matured in TCM-199 medium drops under oil in CO 2/air incubator at 39° C …

In vitro fertilization of pig oocytes after different coincubation intervals

P Coy, E Martinez, S Ruiz, JM Vazquez, J Roca… - Theriogenology, 1993 - Elsevier
The effects of gamete coincubation time on porcine in vitro fertilization for 4, 6 or 8 hours in
Trial 1 or for 1, 2, 3 or 4 hours in Trial 2 were studied. The 876 oocytes were recovered from …

Fertilization and blastocyst development in oocytes obtained from prepubertal and adult pigs

ES Sherrer, TJ Rathbun, DL Davis - Journal of animal science, 2004 - academic.oup.com
Polyspermic fertilization and embryo quality are important issues for the in vitro production of
pig embryos. We hypothesized that oocyte donor (prepubertal gilt vs. sow) affects …

Influence of cumulus cells on in vitro fertilization of bovine oocytes derived from in vitro maturation

RC Chian, K Okuda, K Niwa - Animal Reproduction Science, 1995 - Elsevier
The effect of the cumulus cells on in vitro fertilization (IVF) in cattle was evaluated. Frozen-
thawed bovine spermatozoa were treated with 5 mM caffeine and 10 μg heparin ml− 1 for …

In vitro fertilization of in vitro matured pig oocytes: effects of boar and ejaculate fraction

X Xu, J Ding, PC Seth, DS Harbison, GR Foxcroft - Theriogenology, 1996 - Elsevier
Ejaculates from 3 young boars were collected on 4 occasions as a series of separate 15-ml
fractions. The contribution of different fractions of these ejaculates to observed variability in …