The cultured skin substitute was created through successive cultivation of fibroblasts and
keratinocytes that were combined within a collagen matrix. This collagen matrix was …

Methods for serial cultivation of human keratinocytes can provide large quantities of
epidermal cells, which have the potential of restoring the vital barrier function of the …

We developed a novel artificial skin substitute consisting of two collagen sponge layers with
different pore sizes and cross-link densities. Fibroblasts suspended in 0.5 ml Dulbecco …

Organotypic cultures of human skin were made using dermal fibroblasts seeded into a type I
collagen gel overlaid with epidermal keratinocytes. Full-thickness excision of tattoos was …

We used a living bilayered cultured skin replacement to close full-thickness wound defects
on the dorsum of athymic mice. The skin replacement is composed of human fibroblasts that …

This study compares two techniques for making cultured skin substitutes: a composite graft
made of human fibroblasts and keratinocytes on a collagen-glycosaminoglycan membrane …

The purpose of this study was to examine whether epithelialisation is promoted when an
allogeneic cultured dermal substitute is used as a biological wound dressing. The dermal …

The history of techniques for the replacement of lost skin is reviewed, including the current
research in the use of synthetic dermal substitutes, skin allografts and immunosuppression …

Over the past 2 decades, there has been much research and development of artificial
materials or skin grafts grown in the laboratory for skin replacement. These skin substitutes …

Immediate resurfacing of skin defects is a challenging prospect, especially in patients with
extensive full-thickness burns. Currently, split-thickness autografts offer the best form of …