The development of gene-editing technology holds tremendous potential for accelerating
crop trait improvement to help us address the need to feed a growing global population …

The international floriculture industry is expected to become bigger in the near future, owing
to the continuous increase in the demand for ornamental plants. To facilitate the demand …

Key message We have established a DNA-free genome editing method via
ribonucleoprotein-based CRISPR/Cas9 in cultivated tomato and obtained mutant plants …

Background Plants have a remarkable reprogramming potential, which facilitates plant
regeneration, especially from a single cell. Protoplasts have the ability to form a cell wall and …

Genetic transformation is an important strategy for enhancing plant biomass or resistance in
response to adverse environments and population growth by imparting desirable genetic …

Agriculture is experiencing a technological inflection point in its history, while also facing
unprecedented challenges posed by human population growth and global climate changes …

Plant transformation remains the most sought-after technology for functional genomics and
crop genetic improvement, especially for introducing specific new traits and to modify or …

Protoplasts, which are plant cells with their cell walls removed, have been used for decades
in plant research and have been instrumental in genetic transformation and the study of …

In this review we focus on recent progress in protoplast regeneration, symmetric and
asymmetric hybridization and novel technology developments. Regeneration of new species …

In recent years, plant genetic engineering has advanced agriculture in terms of crop
improvement, stress and disease resistance, and pharmaceutical biosynthesis. Cells from …