Membrane fusion correlates with surface charge in exocytic vesicles

JG Duman, E Lee, GY Lee, G Singh, JG Forte - Biochemistry, 2004 - ACS Publications
Stimulation of gastric parietal cells results in exocytic recruitment of the proton pump (H+, K+-
ATPase) from a pool of intracellular membranes (tubulovesicles) to the apical plasma …

Separation of the osmotically driven fusion event from vesicle-planar membrane attachment in a model system for exocytosis.

MH Akabas, FS Cohen, A Finkelstein - The Journal of cell biology, 1984 - rupress.org
We demonstrate that there are two experimentally distinguishable steps in the fusion of
phospholipid vesicles with planar bilayer membranes. In the first step, the vesicles form a …

Ca2+ and Mg2+/ATP Independently Trigger Homotypic Membrane Fusion in Gastric Secretory Membranes

JG Duman, G Singh, GY Lee, TE Machen, JG Forte - Traffic, 2002 - Wiley Online Library
Exocytic activation of gastric parietal cells represents a massive transformation. We studied
a step in this process, homotypic fusion of H, K‐ATPase‐containing tubulovesicles, using …

Fusion between endocytic vesicles in a cell-free system.

WA Braell - Proceedings of the National Academy of …, 1987 - National Acad Sciences
Cell-free fusion between endocytic vesicles has been obtained in a sensitive assay based
on the avidin-biotin binding reaction. Chinese hamster ovary cells are allowed to …

In vitro fusion of endocytic vesicles: effects of reagents that alter endosomal pH

DD Pless, RB Wellner - Journal of cellular biochemistry, 1996 - Wiley Online Library
Ricin, a plant toxin that binds to galactose‐terminated glycoproteins and glycolipids on the
cell surface, is internalized into endosomes before reaching the cytosol where it exerts its …

[PDF][PDF] Role of channels in the fusion of vesicles with a planar bilayer

DJ Woodbury, JE Hall - Biophysical journal, 1988 - cell.com
Fluorescence microscopy combined with electrical conductance measurements were used
to assess fusion of phospholipid vesicles with a planar bilayer. Large unilamellar vesicles …

Fusion competence of phosphatidylserine-containing liposomes quantitatively measured by a fluorescence resonance energy transfer assay

PS Uster, DW Deamer - Archives of Biochemistry and Biophysics, 1981 - Elsevier
A sensitive, quantitative assay has been developed which measures the extent of liposome
fusion by monitoring fluorescence resonance energy transfer between two lipid analogs …

Effects of divalent ions on vesicle-vesicle fusion studied by a new luminescence assay for fusion

RW Holz, CA Stratford - The Journal of Membrane Biology, 1979 - Springer
A new assay has been developed for vesicle-vesicle fusion based upon the mixing of
intravesicular contents of two sets of vesicles. Purified firefly luciferase and MgCl 2 were …

Influence of phospholipid asymmetry on fusion between large unilamellar vesicles

SJ Eastman, MJ Hope, KF Wong, PR Cullis - Biochemistry, 1992 - ACS Publications
Influence of phospholipid asymmetry on fusion between large unilamellar vesicles Page 1
4262 Biochemistry 1992, 31, 4262-4268 Siegel, R. C., Schumaker, V. N., & Poon, P. H. (1981) …

Osmotic swelling of vesicles: its role in the fusion of vesicles with planar phospholipid bilayer membranes and its possible role in exocytosis

A Finkelstein, J Zimmerberg… - Annual Review of …, 1986 - annualreviews.org
Fusion of intracellular vesicles with the plasma membrane, resulting in the extracellular
discharge (exocytosis) of vesicular contents, plays a prominent role in a plethora of …