Development and viability of in vitro derived porcine blastocysts cultured in NCSU23 and G1. 2/G2. 2 sequential medium

JE Swain, CL Bormann, RL Krisher - Theriogenology, 2001 - Elsevier
Porcine embryo development in vitro is relatively inefficient compared to other domestic
species. Currently, a single culture medium (NCSU23) is the standard for porcine in vitro …

Substrate utilization in porcine embryos cultured in NCSU23 and G1. 2/G2. 2 sequential culture media

AP Gandhi, M Lane, DK Gardner… - Molecular …, 2001 - Wiley Online Library
Embryo metabolism is an indicator of viability and, therefore, efficiency of the culture
medium. Currently, little is known regarding porcine embryo metabolism. The objective of …

Development and quality of porcine embryos in different culture system and embryo-producing methods

SA Ock, SL Lee, JG Kim, BM Kumar… - Zygote, 2007 - cambridge.org
In this study, the developmental ability and cellular composition of porcine IVF, parthenote
and somatic cell nuclear transfer (SCNT) embryos were evaluated following different in vitro …

Effects of fetal calf serum, amino acids, vitamins and insulin on blastocoel formation and hatching of in vivo and IVM/IVF-derived porcine embryos developing in vitro

DB Koo, NH Kim, HT Lee, KS Chung - Theriogenology, 1997 - Elsevier
The objective of this study was to determine the effects of fetal calf serum (FCS), non-
essential MEM amino acids, MEM vitamins and insulin on blastocoel formation, expansion …

In vitro development to blastocysts of early porcine embryos produced in vivo or in vitro

D Rath, H Niemann, CRL Torres - Theriogenology, 1995 - Elsevier
The objective of this study was to compare the development of porcine embryos from the 2-
and 4-cell stages to the blastocyst stage after in vivo or in vitro fertilization and in vivo or in …

The evolution of porcine embryo in vitro production

CG Grupen - Theriogenology, 2014 - Elsevier
The in vitro production of porcine embryos has presented numerous challenges to
researchers over the past four decades. Some of the problems encountered were specific to …

Defined system for in vitro production of porcine embryos using a single basic medium

K Yoshioka, C Suzuki, A Onishi - Journal of Reproduction and …, 2008 - jstage.jst.go.jp
We have previously indicated that porcine blastocysts can be produced by in vitro
fertilization (IVF) and culture (IVC) in chemically defined porcine gamete medium (PGM) and …

Effect of exogenous carbohydrates in a serum-free culture medium on the development of in vitro matured and fertilized porcine embryos

Y Park, J Hong, H Yong, J Lim, E Lee - Zygote, 2005 - cambridge.org
This study was conducted to examine the effect of energy substrates in a serum-free culture
medium on in vitro development of porcine embryos. Presumptive zygotes derived from in …

Effects of culture media and inhibitors on biology of porcine early embryonic development in vitro

H Wang, N Rodriguez-Osorio, JM Feugang, SY Jung… - Livestock Science, 2009 - Elsevier
In vitro-production of porcine embryos is crucial for biomedical and agricultural research,
however, current culture systems for porcine embryos are sub-optimal, and the …

The quality after culture in vitro or in vivo of porcine oocytes matured and fertilized in vitro and their ability to develop to term

Y Nakamura, S Tajima, K Kikuchi - Animal Science Journal, 2017 - Wiley Online Library
The quality of porcine blastocysts produced in vitro is poor in comparison with those that
develop in vivo. We examined the quality of in vitro‐matured and fertilized (IVM/IVF) oocytes …