The side population (SP) is a subpopulation of mouse bone marrow cells highly enriched for
hematopoietic stem cell activity. The SP is identified using flow cytometry as a minor …

Summary The “side population”(SP) phenotype is a manifestation of primitive cells' ability to
efficiently efflux the fluorescent DNA-staining dye Hoechst 33342 and can be used as the …

The protocol for isolation of side population (SP) cells was originally established for murine
bone marrow hematopoietic stem cells (HSCs), but it has also been adapted for other …

The side population (SP) phenotype has been reported as a method to identify
hematopoietic stem cells in the bone marrow based upon differential staining with the …

Hematopoietic stem cells (HSCs) can be isolated from murine bone marrow by their ability to
efflux the Hoechst 33342 dye. This method defines an extremely small and …

A defining property of murine hematopoietic stem cells (HSCs) is low fluorescence after
staining with Hoechst 33342 and Rhodamine 123. These dyes have proven to be …

A novel Hoechst 33342 dye efflux assay was recently developed that identifies a population
of hematopoietic cells termed side population (SP) cells. In the bone marrow of multiple …

We describe the multiparameter flow cytometric analysis of the relationship between side
population (SP) formation and well-characterized, antigen-defined stem cell subsets. We …

Hematopoietic stem cells (HSCs) are defined by the capabilities of multi-lineage
differentiation and long-term self-renewal. Both these characteristics contribute to maintain …

OBJECTIVE: The aim of this study was to characterize murine side population (SP) stem
cells and SP cell subpopulations for primitive stem cell capacity. MATERIALS AND …