Optical mapping and sequencing of the Escherichia coli KO11 genome reveal extensive chromosomal rearrangements, and multiple tandem copies of the …

PC Turner, LP Yomano, LR Jarboe… - Journal of Industrial …, 2012 - academic.oup.com
Abstract Escherichia coli KO11 (ATCC 55124) was engineered in 1990 to produce ethanol
by chromosomal insertion of the Zymomonas mobilis pdc and adhB genes into E. coli W …

A physical map of the genome of ethanol fermentative bacterium Zymomonas mobilis ZM4 and localization of genes on the map

HL Kang, HS Kang - Gene, 1998 - Elsevier
A physical map of the Zymomonas mobilis ZM4 genome has been constructed from the
results of reciprocal Southern hybridization with PmeI, PacI, and NotI-digested genomic DNA …

Expression of a Lactose Transposon (Tn951) in Zymomonas mobilis

VC Carey, SK Walia, LO Ingram - Applied and environmental …, 1983 - Am Soc Microbiol
The potential utility of Zymomonas mobilis as an organism for the commercial production of
ethanol would be greatly enhanced by the addition of foreign genes which expand its range …

R-Plasmid Transfer in Zymomonas mobilis

ML Skotnicki, DE Tribe, PL Rogers - Applied and Environmental …, 1980 - Am Soc Microbiol
Conjugal transfer of three IncP1 plasmids and one IncFII plasmid into strains of the ethanol-
producing bacterium Zymomonas mobilis was obtained. These plasmids were transferred at …

Biosynthetic Burden and Plasmid Burden Limit Expression of Chromosomally Integrated Heterologous Genes (pdc, adhB) in Escherichia coli

A Martinez, SW York, LP Yomano… - Biotechnology …, 1999 - Wiley Online Library
Previous studies have shown an unexpectedly high nutrient requirement for efficient ethanol
production by ethanologenic recombinants of Escherichia coli B such as LY01 which contain …

Chromosomal Integration of Heterologous DNA in Escherichia coli with Precise Removal of Markers and Replicons Used during Construction

F Martinez-Morales, AC Borges, A Martinez… - Journal of …, 1999 - Am Soc Microbiol
ABSTRACT A set of vectors which facilitates the sequential integration of new functions into
the Escherichia coli chromosome by homologous recombination has been developed …

Comparative proteomic and genetic analyses reveal unidentified mutations in Escherichia coli XL1-Blue and DH5α

XX Xia, ZG Qian, SY Lee - FEMS microbiology letters, 2011 - academic.oup.com
Escherichia coli has been used widely in laboratory and the biotech industry. However, the
genetic and metabolic characteristics remain inadequately studied, particularly for those …

Deletion of pyruvate decarboxylase gene in Zymomonas mobilis by recombineering through bacteriophage lambda red genes

R Khandelwal, S Agrawal, D Singhi… - Journal of …, 2018 - Elsevier
Zymomonas mobilis ZM4 is a gram negative ethanologenic bacterium used in several
biotechnological applications. Metabolic engineering in this bacterium is limited because of …

The Complete Genome Sequence of Escherichia coli DH10B: Insights into the Biology of a Laboratory Workhorse

T Durfee, R Nelson, S Baldwin, G Plunkett III… - Journal of …, 2008 - Am Soc Microbiol
Escherichia coli DH10B was designed for the propagation of large insert DNA library clones.
It is used extensively, taking advantage of properties such as high DNA transformation …

Linkage Map of Escherichia coli K-12, Edition 10: The Physical Map

KE Rudd - Microbiology and molecular biology reviews, 1998 - Am Soc Microbiol
SUMMARY A physical map, EcoMap10, of the now completely sequenced Escherichia coli
chromosome is presented. Calculated genomic positions for the eight restriction enzymes …