Effect of different protein supplements on motility and plasma membrane integrity of frozen-thawed stallion spermatozoa

J Braun, S Hochi, N Oguri, K Sato, F Torres-Boggino - Cryobiology, 1995 - Elsevier
Three experiments were conducted to evaluate the effect of different macromolecule
components (egg yolk, skim milk, and BSA) in a widely employed extender for …

Cooling and freezing of boar spermatozoa: supplementation of the freezing media with reduced glutathione preserves sperm function

J Gadea, F García‐Vazquez, C Matás… - Journal of …, 2005 - Wiley Online Library
In this study, we evaluated the effects of glutathione (l‐γ‐glutamyl‐l‐cysteinylglycine; GSH)
supplementation of the freezing extender on semen parameters during the cooling (2 hours …

Membrane integrity, mitochondrial activity, ATP content, and motility of the European catfish (Silurus glanis) testicular spermatozoa after freezing with different …

BO De Baulny, C Labbé, G Maisse - Cryobiology, 1999 - Elsevier
The extent of cellular damage was investigated after freeze–thawing of the European catfish
testicular sperm with various cryoprotectants. The best protection was given by …

Thawing and processing of cryopreserved bovine spermatozoa at various temperatures and their effects on sperm viability, osmotic shock and sperm membrane …

JR Correa, MC Rodriguez, DJ Patterson, PM Zavos - Theriogenology, 1996 - Elsevier
The objective of this study was to evaluate the effects of thawing and processing
temperatures on post-thaw sperm viability, occurrence of osmotic shock and sperm …

Cryopreservation of European eel (Anguilla anguilla) spermatozoa: effect of dilution ratio, foetal bovine serum supplementation, and cryoprotectants

F Marco-Jiménez, DL Garzón, DS Peñaranda, L Pérez… - Cryobiology, 2006 - Elsevier
The main aim of the present study was to investigate the effect of sperm freezing medium
dilution ratio (1: 1, 1: 2, and 1: 5v/v), two cryoprotectants: dimethyl sulphoxide (Me2SO) and …

Seminal plasma applied post-thawing affects boar sperm physiology: A flow cytometry study

R Fernández-Gago, JC Domínguez, F Martínez-Pastor - Theriogenology, 2013 - Elsevier
Cryopreservation induces extensive biophysical and biochemical changes in the sperm. In
the present study, we used flow cytometry to assess the capacitation-like status of frozen …

Effect of extender composition and equilibration time on fertilization ability and enzymatic activity of rainbow trout cryopreserved spermatozoa

I Babiak, J Glogowski, K Goryczko, S Dobosz… - Theriogenology, 2001 - Elsevier
The effects of extender composition and equilibration time on fertilizing ability of
cryopreserved spermatozoa from rainbow trout, Oncorhynchus mykiss, were investigated. In …

Flow cytometric evaluation of mitochondrial activity and membrane integrity in fresh and cryopreserved rainbow trout (Oncorhynchus mykiss) spermatozoa

BO De Baulny, Y Le Vern, D Kerboeuf, G Maisse - Cryobiology, 1997 - Elsevier
There are still several obstacles to effective cryopreservation of trout spermatozoa as seen
by the large variability of fertility rates obtained with frozen–thawed spermatozoa. We …

Effects of bovine sperm cryopreservation using different freezing techniques and cryoprotective agents on plasma, acrosomal and mitochondrial membranes

RA Forero‐Gonzalez, ECC Celeghini, CF Raphael… - Andrologia, 2012 - Wiley Online Library
The success of semen cryopreservation is influenced by several factors, such as freezing
curves and cryoprotectants. These two factors are of special interest once they may lead to …

Membrane changes during different stages of a freeze–thaw protocol for equine semen cryopreservation

DM Neild, BM Gadella, MG Chaves, MH Miragaya… - Theriogenology, 2003 - Elsevier
Many theories have been postulated concerning the possible effects of cryopreservation on
spermatozoa, including suggestions the freeze–thawing process produces membranes that …