Efficient CRISPR-Cas9 based cytosine base editors for phytopathogenic bacteria

C Li, L Wang, LJ Cseke, F Vasconcelos… - Communications …, 2023 - nature.com
Phytopathogenic bacteria play important roles in plant productivity, and developments in
gene editing have potential for enhancing the genetic tools for the identification of critical …

Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor

F Veillet, L Perrot, L Chauvin, MP Kermarrec… - International Journal of …, 2019 - mdpi.com
Genome editing tools have rapidly been adopted by plant scientists for gene function
discovery and crop improvement. The current technical challenge is to efficiently induce …

CRISPR-Assisted Multiplex Base Editing System in Pseudomonas putida KT2440

J Sun, LB Lu, TX Liang, LR Yang… - Frontiers in Bioengineering …, 2020 - frontiersin.org
Pseudomonas putida (P. putida) KT2440 is a paradigmatic environmental-bacterium that
possesses significant potential in synthetic biology, metabolic engineering and …

New variants of CRISPR RNA‐guided genome editing enzymes

J Murovec, Ž Pirc, B Yang - Plant biotechnology journal, 2017 - Wiley Online Library
CRISPR‐mediated genome editing using the Streptococcus pyogenes Cas9 enzyme is
revolutionizing life science by providing new, precise, facile and high‐throughput tools for …

CRISPR/Cas9‐based genome editing and its applications for functional genomic analyses in plants

J Li, Y Li, L Ma - Small Methods, 2019 - Wiley Online Library
The ability to modify complex genomes precisely to create specific mutants is the holy grail
of basic research and applied genetics in the postgenomic era. Programmable sequence …

Expanding the plant genome editing toolbox with recently developed CRISPR–Cas systems

N Wada, K Osakabe, Y Osakabe - Plant Physiology, 2022 - academic.oup.com
Since its first appearance, CRISPR–Cas9 has been developed extensively as a
programmable genome-editing tool, opening a new era in plant genome engineering …

[HTML][HTML] CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants

Z Jiang, X Hong, S Zhang, R Yao, YI Xiao - Synthetic and Systems …, 2020 - Elsevier
Abstract CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-
CRISPR associated) has been extensively exploited as a genetic tool for genome editing …

DNA-free genome editing: past, present and future

J Metje-Sprink, J Menz, D Modrzejewski… - Frontiers in Plant …, 2019 - frontiersin.org
Genome Editing using engineered endonuclease (GEEN) systems rapidly took over the field
of plant science and plant breeding. So far, Genome Editing techniques have been applied …

Targeted plant genome editing via the CRISPR/Cas9 technology

JF Li, D Zhang, J Sheen - Plant Functional Genomics: Methods and …, 2015 - Springer
Targeted modification of plant genome is key for elucidating and manipulating gene
functions in basic and applied plant research. The CRISPR (clustered regularly interspaced …

CRISPR/Cas-mediated base editing: technical considerations and practical applications

KA Molla, Y Yang - Trends in biotechnology, 2019 - cell.com
Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new
CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or …