Determination of genomic 5-hydroxymethyl-2'-deoxycytidine in human DNA by capillary electrophoresis with laser induced fluorescence
Recent studies reported the presence of 5-hydroxymethylcytosine (5 hmC) as an additional
modification in mammalian genomic DNA. To date, 5 hmC has been detected only in mouse …
modification in mammalian genomic DNA. To date, 5 hmC has been detected only in mouse …
Spectroscopic quantification of 5-hydroxymethylcytosine in genomic DNA
5-Hydroxymethylcytosine (5hmC), a modified form of the DNA base cytosine, is an important
epigenetic mark linked to regulation of gene expression in development, and tumorigenesis …
epigenetic mark linked to regulation of gene expression in development, and tumorigenesis …
Determination of 5-methyl-2′-deoxycytidine in genomic DNA using high performance liquid chromatography-ultraviolet detection
J Sandhu, B Kaur, C Armstrong, CJ Talbot… - … of Chromatography B, 2009 - Elsevier
The formation of 5-methyl-2′-deoxycytidine (5-MedC) following methylation of the C-5
position of cytosine in genomic DNA provides an epigenetic mechanism for the regulation of …
position of cytosine in genomic DNA provides an epigenetic mechanism for the regulation of …
A method to assess genomic DNA methylation using high-performance liquid chromatography/electrospray ionization mass spectrometry
Eukaryotic DNA is methylated at some cytosine residues, and this epigenetic feature
performs critical functions. We developed a method for quantitative determination of 5 …
performs critical functions. We developed a method for quantitative determination of 5 …
Single-molecule quantification of 5-hydroxymethylcytosine for diagnosis of blood and colon cancers
Background The DNA modification 5-hydroxymethylcytosine (5hmC) is now referred to as
the sixth base of DNA with evidence of tissue-specific patterns and correlation with gene …
the sixth base of DNA with evidence of tissue-specific patterns and correlation with gene …
A validated quantitative liquid chromatography–tandem quadrupole mass spectrometry method for monitoring isotopologues to evaluate global modified cytosine …
M Tsuji, H Matsunaga, D Jinno, H Tsukamoto… - … of Chromatography B, 2014 - Elsevier
Hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC) represent important epigenetic
modifications to DNA, and a sensitive analytical method is required to determine the levels …
modifications to DNA, and a sensitive analytical method is required to determine the levels …
A novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNA
Abstract Recently, 5-hydroxymethylcytosine (5hmC) was identified in mammalian genomic
DNA. The biological role of this modification remains unclear; however, identifying the …
DNA. The biological role of this modification remains unclear; however, identifying the …
Hydroxymethylated DNA immunoprecipitation (hmeDIP)
hydroxymethylcytosine (5hmC) was recently identified as an abundant epigenetic mark in
mammals. Subsequent research has implicated 5hmC in normal mammalian development …
mammals. Subsequent research has implicated 5hmC in normal mammalian development …
Epigenetic Quantification of DNA 5‐Hydroxymethylcytosine Using DNA Hybridization‐Based Single‐Molecule Immunofluorescent Imaging
Y Du, Y Lai, JY Liu, J Diao - Small Methods, 2021 - Wiley Online Library
Hydroxymethylcytosine (5hmC) is a deoxyribonucleic acid (DNA) epigenetic modification
that has an important function in embryonic development and human diseases. However …
that has an important function in embryonic development and human diseases. However …
A sensitive mass spectrometry method for simultaneous quantification of DNA methylation and hydroxymethylation levels in biological samples
T Le, KP Kim, G Fan, KF Faull - Analytical biochemistry, 2011 - Elsevier
The recent discovery of 5-hydroxymethyl-cytosine (5hmC) in embryonic stem cells and
postmitotic neurons has triggered the need for quantitative measurements of both 5-methyl …
postmitotic neurons has triggered the need for quantitative measurements of both 5-methyl …