Expression of the TOL plasmid xylS gene in Pseudomonas putida occurs from a alpha 70-dependent promoter or from alpha 70-and alpha 54-dependent tandem …

MT Gallegos, S Marqués, JL Ramos - Journal of bacteriology, 1996 - Am Soc Microbiol
Growth of Pseudomonas putida (pWWO) on alkylbenzoates requires the expression of the
meta pathway operon, which is mediated by the XylS protein after binding of a benzoate …

[HTML][HTML] Synthetic vanillate-regulated promoter for graded gene expression in Sphingomonas

A Kaczmarczyk, JA Vorholt, A Francez-Charlot - Scientific reports, 2014 - nature.com
Regulated promoters are an important basic genetic tool allowing, for example, gene-
dosage and gene depletion studies. We have previously described a cumate-inducible …

An upstream XylR‐and IHF‐induced nucleoprotein complex regulates the sigma 54‐dependent Pu promoter of TOL plasmid.

V de Lorenzo, M Herrero, M Metzke… - The EMBO Journal, 1991 - embopress.org
Transcription from promoter Pu of the upper catabolic operon of the Pseudomonas putida
TOL plasmid which specifies conversion of toluene/xylenes to benzoate/toluates is activated …

[HTML][HTML] Recruitment of σ54-RNA polymerase to the Pu promoter of Pseudomonas putida through integration host factor-mediated positioning switch of α subunit …

R Macchi, L Montesissa, K Murakami… - Journal of Biological …, 2003 - ASBMB
The interactions between the σ 54-containing RNA polymerase (σ 54-RNAP) and the region
of the Pseudomonas putida Pu promoter spanning from the enhancer to the binding site for …

Identification of a cis-acting sequence within the Pm promoter of the TOL plasmid which confers XylS-mediated responsiveness to substituted benzoates

B Kessler, V de Lorenzo, KN Timmis - Journal of molecular biology, 1993 - Elsevier
The DNA sequences within the Pm promoter/operator region of the meta operon of the TOL
plasmid of Pseudomonas putida, which confer XylS-mediated responsiveness to substituted …

The organization of the Pm promoter of the TOL plasmid reflects the structure of its cognate activator protein XylS

B Kessler, KN Timmis, V de Lorenzo - Molecular and General Genetics …, 1994 - Springer
The toluate catabolic operon carried by the TOL plasmid pWWO of Pseudomonas putida is
positively regulated by the benzoate-responsive XylS protein which, when activated …

[HTML][HTML] σ54-promoter discrimination and regulation by ppGpp and DksA

LMD Bernardo, LUM Johansson, E Skarfstad… - Journal of biological …, 2009 - ASBMB
The σ 54-factor controls expression of a variety of genes in response to environmental cues.
Much previous work has implicated the nucleotide alarmone ppGpp and its co-factor DksA in …

[HTML][HTML] Recruitment of RNA Polymerase Is a Rate-limiting Step for the Activation of the ς54 Promoter Pu ofPseudomonas putida

M Carmona, V de Lorenzo, G Bertoni - Journal of Biological Chemistry, 1999 - ASBMB
The activity of the ς 54-promoter Pu of Pseudomonas putida was examined in vitro with a
DNA template lacking upstream activating sequences, such that RNA polymerase can be …

Growth-phase-dependent expression of the Pseudomonas putida TOL plasmid pWW0 catabolic genes

N Hugouvieux-Cotte-Pattat, T Köhler… - Journal of …, 1990 - Am Soc Microbiol
Pseudomonas putida TOL plasmid pWW0 catabolic genes are clustered into two operons.
The first, the upper operon, is controlled by the xylR regulatory gene, whereas the second …

Visualization of DNA–protein intermediates during activation of the Pu promoter of the TOL plasmid of Pseudomonas putida

J Garmendia, V de Lorenzo - Microbiology, 2000 - microbiologyresearch.org
The ATP-dependent multimerization process undergone by the σ54-dependent activator
XylR of the TOL plasmid pWW0 of Pseudomonas putida when bound to the upstream …