Systematic discovery of natural CRISPR-Cas12a inhibitors
Cas12a (Cpf1) is a CRISPR-associated nuclease with broad utility for synthetic genome
engineering, agricultural genomics, and biomedical applications. Although bacteria …
engineering, agricultural genomics, and biomedical applications. Although bacteria …
Genome editing by natural and engineered CRISPR-associated nucleases
Over the last decade, research on distinct types of CRISPR systems has revealed many
structural and functional variations. Recently, several novel types of single-polypeptide …
structural and functional variations. Recently, several novel types of single-polypeptide …
[HTML][HTML] Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system
B Zetsche, JS Gootenberg, OO Abudayyeh… - cell, 2015 - cell.com
The microbial adaptive immune system CRISPR mediates defense against foreign genetic
elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize …
elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize …
Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting
F Chen, X Ding, Y Feng, T Seebeck, Y Jiang… - Nature …, 2017 - nature.com
Bacterial CRISPR–Cas systems comprise diverse effector endonucleases with different
targeting ranges, specificities and enzymatic properties, but many of them are inactive in …
targeting ranges, specificities and enzymatic properties, but many of them are inactive in …
Discovery of potent and versatile CRISPR–Cas9 inhibitors engineered for chemically controllable genome editing
Anti-CRISPR (Acr) proteins are encoded by many mobile genetic elements (MGEs) such as
phages and plasmids to combat CRISPR–Cas adaptive immune systems employed by …
phages and plasmids to combat CRISPR–Cas adaptive immune systems employed by …
Endogenous CRISPR-Cas system-based genome editing and antimicrobials: review and prospects
Y Li, N Peng - Frontiers in microbiology, 2019 - frontiersin.org
CRISPR-Cas systems adapt “memories” via spacers from viruses and plasmids to develop
adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR …
adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR …
The Cpf1 CRISPR-Cas protein expands genome-editing tools
The Cpf1 CRISPR-Cas protein expands genome-editing tools | Genome Biology Skip to main
content SpringerLink Account Menu Find a journal Publish with us Track your research Search …
content SpringerLink Account Menu Find a journal Publish with us Track your research Search …
The CRISPR tool kit for genome editing and beyond
M Adli - Nature communications, 2018 - nature.com
CRISPR is becoming an indispensable tool in biological research. Once known as the
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …
The revolution continues: newly discovered systems expand the CRISPR-Cas toolkit
CRISPR-Cas systems defend prokaryotes against bacteriophages and mobile genetic
elements and serve as the basis for revolutionary tools for genetic engineering. Class 2 …
elements and serve as the basis for revolutionary tools for genetic engineering. Class 2 …
Miniature type VF CRISPR-Cas nucleases enable targeted DNA modification in cells
G Bigelyte, JK Young, T Karvelis, K Budre… - Nature …, 2021 - nature.com
Class 2 CRISPR systems are exceptionally diverse, nevertheless, all share a single effector
protein that contains a conserved RuvC-like nuclease domain. Interestingly, the size of these …
protein that contains a conserved RuvC-like nuclease domain. Interestingly, the size of these …