The mouse α-lactalbumin gene has been replaced with the human gene by two consecutive
rounds of gene targeting in hypoxanthine phosphoribosyltransferase (HPRT)-deficient …

We describe a two-step strategy to alter any mouse locus repeatedly and efficiently by direct
positive selection. Using conventional targeting for the first step, a functional neo gene and a …

Mutations were targeted to the Hprt locus in murine embryonic stem cells by using sequence
replacement vectors. When the vector was designed such that the mutated sequences were …

We have introduced a 4-bp insertion into the hypoxanthine phosphoribosyltransferase
(HPRT) gene of a mouse embryonic stem (ES) cell line by using an “in-out” targeting …

The hypoxanthine phosphoribosyltransferase (Hprt) locus has been shown to have minimal
influence on transgene expression when used as a surrogate site in the mouse genome. We …

We mutated, by gene targeting, the endogenous hypoxanthine phosphorlbosyi transferase
(HPFlT) gene in mouse embryo-derived stem (ES) cells. A specialized construct of the …

The use ofgene targeting in embryonic stem (ES) cells provides an attractive method for the
introduction of genetic alterations into the mammalian germ line (for review see 1). For this …

A convenient system for gene targeting that uses hypoxanthine phosphoribosyltransferase
(HPRT) minigenes as the selectable marker in HPRT-deficient mouse embryonic stem (ES) …

The rat is the preferred experimental animal in many biological studies. With the recent
derivation of authentic rat embryonic stem (ES) cells it is now feasible to apply state-of-the …

Mutations were targeted to the Hprt locus of mouse embryo-derived stem cells by using 22
different sequence replacement and sequence insertion vectors. The targeting frequency …