The crystal structure of the membrane-bound O2-tolerant [NiFe]-hydrogenase 1 from
Escherichia coli (Ec Hyd-1) has been solved in three different states: as-isolated, H2 …

Hydrogenases, abundant proteins in the microbial world, catalyze cleavage of H 2 into
protons and electrons or the evolution of H 2 by proton reduction. Hydrogen metabolism …

Iron-sulfur clusters are versatile electron transfer cofactors, ubiquitous in metalloenzymes
such as hydrogenases. In the oxygen-tolerant Hydrogenase I from Aquifex aeolicus such …

Clostridium acetobutylicum ATCC 824 was selected for the homologous overexpression of
its Fe-only hydrogenase and for the heterologous expressions of the Chlamydomonas …

The iron− sulfur cluster-free hydrogenase (Hmd) from methanogenic archaea harbors an
iron-containing, light-sensitive cofactor of still unknown structure as prosthetic group. The …

The reaction takes place at a specialized metal center that dramatically increases the acidity
of H2 and leads to a heterolytic splitting of the molecule which is strongly accelerated by the …

Two energy-generating hydrogenases enable the aerobic hydrogen bacterium Ralstonia
eutropha (formerly Alcaligenes eutrophus) to use molecular hydrogen as the sole energy …

Hydrogenases represent a heterogeneous group of enzymes consisting of three
evolutionary unrelated classes, ie [NiFe],[FeFe] and [Fe] hydrogenases. They allow the uni …

A third hydrogenase was recently identified in the proteobacterium Alcaligenes eutrophus as
a constituent of a novel H2-sensing multicomponent regulatory system. This regulatory …

Abstract Membrane-bound respiratory [NiFe]-hydrogenase (MBH), a H2-uptake enzyme
found in the periplasmic space of bacteria, catalyses the oxidation of dihydrogen: H2→ …