Biocatalytic ketone reduction: a green and efficient access to enantiopure alcohols
Y Ni, JH Xu - Biotechnology advances, 2012 - Elsevier
Chiral secondary alcohols play an important role in pharmaceutical, agrochemical, and
chemical industries. In recent years, impressive steps forward have been achieved towards …
chemical industries. In recent years, impressive steps forward have been achieved towards …
Comparison of engineered Saccharomyces cerevisiae and engineered Escherichia coli for the production of an optically pure keto alcohol
N Skorupa Parachin, M Carlquist… - Applied microbiology …, 2009 - Springer
In this study, the production of enantiomerically pure (1 R, 4 S, 6 S)-6-hydroxy-bicyclo [2.2. 2]
octane-2-one ((−)-2) through stereoselective bioreduction was used as a model reaction for …
octane-2-one ((−)-2) through stereoselective bioreduction was used as a model reaction for …
Synthesis of both enantiomers of ethyl-4-chloro-3-hydroxbutanoate from a prochiral ketone using Candida parapsilosis ATCC 7330
T Kaliaperumal, SN Gummadi, A Chadha - Tetrahedron: Asymmetry, 2011 - Elsevier
Candida parapsilosis ATCC 7330 when grown in a medium containing glycerol reduced
ethyl-4-chloro-3-oxobutanoate to (R)-ethyl-4-chloro-3-hydroxybutanote (ee> 99%, yield …
ethyl-4-chloro-3-oxobutanoate to (R)-ethyl-4-chloro-3-hydroxybutanote (ee> 99%, yield …
Mechanistic investigation of the Candida albicans CCT 0776 stereoinversion system and application to obtain enantiopure secondary alcohols
SM Mantovani, CFF Angolini, AJ Marsaioli - Tetrahedron: Asymmetry, 2009 - Elsevier
Phenylethanol deracemization with Candida albicans CCT 0776 whole cells yields the (R)-
enantiomer in over 99% enantiomeric excess and 98% yield. The deracemization process …
enantiomer in over 99% enantiomeric excess and 98% yield. The deracemization process …
Significantly improved thermostability of a reductase CgKR1 from Candida glabrata with a key mutation at Asp 138 for enhancing bioreduction of aromatic α-keto …
L Huang, JH Xu, HL Yu - Journal of Biotechnology, 2015 - Elsevier
The keto ester reductase from Candida glabrata, designated as Cg KR1, is a highly versatile
biocatalyst with a broad substrate spectrum. Its substrate preference was altered by rational …
biocatalyst with a broad substrate spectrum. Its substrate preference was altered by rational …
[HTML][HTML] Candida tropicalis CE017: a new Brazilian enzymatic source for the bioreduction of aromatic prochiral ketones
GAB Vieira, DM Araujo, TLG Lemos… - Journal of the Brazilian …, 2010 - SciELO Brasil
The reactivity and stereoselectivity showed by a new strain of Candida tropicalis in the
reduction of prochiral ketones have been compared with the ones previously attained in our …
reduction of prochiral ketones have been compared with the ones previously attained in our …
Highly efficient synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate and its derivatives by a robust NADH-dependent reductase from E. coli CCZU-K14
YC He, ZC Tao, X Zhang, ZX Yang, JH Xu - Bioresource technology, 2014 - Elsevier
An NADH-dependent reductase (CmCR) from Candida magnoliae was discovered by
genome mining for carbonyl reductases. After CmCR was overexpressed in Escherichia coli …
genome mining for carbonyl reductases. After CmCR was overexpressed in Escherichia coli …
Organic transformations catalyzed by engineered yeast cells and related systems
JD Stewart - Current Opinion in Biotechnology, 2000 - Elsevier
Asymmetric ketone reductions remain the most popular application of baker's yeast
(Saccharomyces cerevisiae) in organic synthesis and data from the genome sequencing …
(Saccharomyces cerevisiae) in organic synthesis and data from the genome sequencing …
[HTML][HTML] A Novel Whole-Cell Biocatalyst with NAD+ Regeneration for Production of Chiral Chemicals
Z Xiao, C Lv, C Gao, J Qin, C Ma, Z Liu, P Liu, L Li… - PloS one, 2010 - journals.plos.org
Background The high costs of pyridine nucleotide cofactors have limited the applications of
NAD (P)-dependent oxidoreductases on an industrial scale. Although NAD (P) H …
NAD (P)-dependent oxidoreductases on an industrial scale. Although NAD (P) H …
Improved synthesis of chiral alcohols with Escherichia coli cells co-expressing pyridine nucleotide transhydrogenase, NADP+-dependent alcohol dehydrogenase and …
A Weckbecker, W Hummel - Biotechnology letters, 2004 - Springer
Recombinant pyridine nucleotide transhydrogenase (PNT) from Escherichia coli has been
used to regenerate NAD+ and NADPH. The pnta and pntb genes encoding for the α-and β …
used to regenerate NAD+ and NADPH. The pnta and pntb genes encoding for the α-and β …