Localized sets of random point mutations generated by PCR amplification can be transferred
efficiently to the chromosome of Acinetobacter ADP1 (also known as strain BD413) by …

Acinetobacter sp. strain ADP1 is a naturally transformable gram-negative bacterium with
simple culture requirements, a prototrophic metabolism and a compact genome of 3.7 Mb …

The genomes of most bacteria contain mobile DNA elements that can contribute to
undesirable genetic instability in engineered cells. In particular, transposable insertion …

For many years, the natural competency of Acinetobacter baylyi ADP1 facilitated studies of
bacterial metabolism, biochemistry, and physiology. With the advent of synthetic biology …

A system for studying gene amplification in the bacterium Acinetobacter sp. strain ADP1 was
used to isolate 105 spontaneous mutants. The method selects for the elevated expression of …

In Acinetobacter sp. ADP1, growth on benzoate requires regulation of the cat genes by two
transcriptional activators. Here, mutants were obtained from a strain lacking both activators …

A strain of Acinetobacter calcoaceticus that demonstrates unusually high competence for
natural transformation by linear DNA has proven valuable for analysis of genes and gene …

Mutant analysis is a powerful tool in the study of biological processes, the more so if it is
combined with the relevant physiological experiments. Two basically different approaches …

We describe a general method for random mutagenesis of cloned genes by error-prone
PCR or DNA shuffling that eliminates the need for post-amplification subcloning following …

Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous
recombination, so it can be transformed without restriction digests or ligation reactions …