Transformation of Acinetobacter sp. BD413 with DNA from commercially available genetically modified potato and papaya
M Iwaki, Y Arakawa - Letters in applied microbiology, 2006 - academic.oup.com
Aim: To estimate the likelihood of transfer of kanamycin‐resistance gene (nptII) from
commercially available genetically modified (GM) plants. Methods and Results …
commercially available genetically modified (GM) plants. Methods and Results …
Genetic tools for reliable gene expression and recombineering in Pseudomonas putida
TB Cook, JM Rand, W Nurani… - Journal of Industrial …, 2018 - academic.oup.com
Pseudomonas putida is a promising bacterial host for producing natural products, such as
polyketides and nonribosomal peptides. In these types of projects, researchers need a …
polyketides and nonribosomal peptides. In these types of projects, researchers need a …
Identification and characterization of IS 1411, a new insertion sequence which causes transcriptional activation of the phenol degradation genes in Pseudomonas …
A Kallastu, R Hõrak, M Kivisaar - Journal of bacteriology, 1998 - Am Soc Microbiol
ABSTRACT A new insertion sequence (IS element), IS 1411, was identified downstream of
the phenol degradation genes pheBA that originated from plasmid DNA of Pseudomonas …
the phenol degradation genes pheBA that originated from plasmid DNA of Pseudomonas …
Reversal by DNA amplifications of an unusual mutation blocking alkane and alcohol utilization in Pseudomonas putida
DL McBeth, JA Shapiro - Molecular and General Genetics MGG, 1984 - Springer
We analyzed the reversion of strains carrying alk208, a mutation in the alkBAC (alkane
utilization) region of the Pseudomonas CAM-OCT plasmid. Reversion of alk208 was …
utilization) region of the Pseudomonas CAM-OCT plasmid. Reversion of alk208 was …
RP4 fertility variants in Acinetobacter calcoaceticus
KJ Towner, A Vivian - Genetics Research, 1976 - cambridge.org
The resistance plasmid RP4 can be used to mediate conjugation in Acinetobacter
calcoaceticus. Transfer of chromosomal genes occurs only on solid surfaces and not in …
calcoaceticus. Transfer of chromosomal genes occurs only on solid surfaces and not in …
Blue/white screening of recombinant plasmids in Gram-positive bacteria by interruption of alkaline phosphatase gene (phoZ) expression
DO Chaffin, CE Rubens - Gene, 1998 - Elsevier
The process of screening bacterial transformants for recombinant plasmids is made more
rapid and simple by the use of vectors with visually detectable reporter genes. In such …
rapid and simple by the use of vectors with visually detectable reporter genes. In such …
Random mutagenesis using a mutator strain
Random mutagenesis is a useful technique to study the functions of different gene products.
Propagation of the genes cloned in plasmids through a mutator strain, like Escherichia coli …
Propagation of the genes cloned in plasmids through a mutator strain, like Escherichia coli …
SOE-LRed: A simple and time-efficient method to localize genes with point mutations onto the Escherichia coli chromosome
RW Benson, TM Cafarelli, VG Godoy - Journal of microbiological methods, 2011 - Elsevier
We use a powerful method to replace wild-type genes on the chromosome of Escherichia
coli. Using a unique form of PCR, we generate easily constructible gene fusions bearing …
coli. Using a unique form of PCR, we generate easily constructible gene fusions bearing …
Use of a transposon-encoded site-specific resolution system for construction of large and defined deletion mutations in bacterial chromosome
M Tsuda - Gene, 1998 - Elsevier
A class II transposon, Tn1722, encodes a site-specific resolution system, in which the
resolvase (TnpR) efficiently catalyzes intramolecular recombination between the two directly …
resolvase (TnpR) efficiently catalyzes intramolecular recombination between the two directly …
A comparison of the survival of stable and unstable chromosomally located xylE marker cassettes as an indicator of cell division within populations of Pseudomonas …
C Winstanley, M Seasman, RW Pickup, JP Carter… - 1993 - cabidigitallibrary.org
Delivery systems were constructed to introduce xylE marker cassettes into the chromosome
of target strs of Pseudomonas putida. A Tn5-based method, used to introduce ap R-XYLE-c …
of target strs of Pseudomonas putida. A Tn5-based method, used to introduce ap R-XYLE-c …