Autotransporters constitute a large family of natural proteins that are essential for delivering
many types of proteins and peptides across the outer membrane in Gram-negative bacteria …

Background Cell display technologies (eg bacterial display) are attractive in directed
evolution as they provide the option to use flow-cytometric cell sorting for selection from …

Highlights•Autotransporters have predominantly been used for surface display on E.
coli.•For industrial applications, host bacteria with specific features are needed.•Here we …

To display a protein or peptide with a distinct function at the surface of a living bacterial cell
is a challenging exercise with constantly increasing impact in many areas of biochemistry …

Development of new affinity proteins using combinatorial protein engineering is today
established for generation of monoclonal antibodies and also essential for discovery of …

Escherichia coli is still a very popular host for the production of recombinant proteins at an
analytical or industrial scale. Secretion of the proteins into the culture medium or display at …

Expressing proteins of interest as fusions to proteins of the bacterial envelope is a powerful
technique with many biotechnological and medical applications. Autotransporters have …

Autotransporters (ATs) are a family of bacterial proteins containing a C-terminal β-barrel-
forming domain that facilitates the translocation of N-terminal passenger domain whose …

Autotransporters have been employed as the anchoring scaffold for cell surface display by
replacing their passenger domains with heterologous proteins to be displayed. We adopted …

Background Bacterial surface display is of interest in many applications, including live
vaccine development, screening of protein libraries and the development of whole cell …