Development and validation of a highly efficient protocol of porcine somatic cloning using preovulatory embryo transfer in peripubertal gilts
B Petersen, A Lucas-Hahn, M Oropeza… - Cloning and stem …, 2008 - liebertpub.com
The efficiency of porcine somatic nuclear transfer (born piglets/transferred embryos) is low.
Here, we report a highly efficient protocol using peripubertal gilts as recipients synchronized …
Here, we report a highly efficient protocol using peripubertal gilts as recipients synchronized …
Increasing efficiency in production of cloned piglets
The low efficiency in obtaining piglets after production of cloned embryos was challenged in
two steps—first by performing in vitro culture for 5–6 days after cloning to obtain later-stage …
two steps—first by performing in vitro culture for 5–6 days after cloning to obtain later-stage …
Efficiency of porcine somatic cell nuclear transfer–a retrospective study of factors related to embryo recipient and embryos transferred
Y Huang, H Ouyang, H Yu, L Lai, D Pang… - Biology Open, 2013 - journals.biologists.com
The successful generation of pigs via somatic cell nuclear transfer depends on reducing risk
factors in several aspects. To provide an overview of some influencing factors related to …
factors in several aspects. To provide an overview of some influencing factors related to …
Cloned Piglets Born after Nuclear Transplantation of Embryonic Blastomeres into Porcine Oocytes Matured In Vivo
GP Li, JH Tan, QY Sun, QG Meng, KZ Yue, XS Sun… - Cloning, 2000 - liebertpub.com
Nuclear transplantation in the pig is more difficult than in other domestic animals and only
one embryonic nuclear transplantation (NT) pig has been born to date. In this study …
one embryonic nuclear transplantation (NT) pig has been born to date. In this study …
A Highly Efficient Method for Porcine Cloning by Nuclear Transfer Using In Vitro-Matured Oocytes
SC Walker, T Shin, GM Zaunbrecher… - Cloning & Stem …, 2002 - liebertpub.com
To date, the efficiency of pig cloning by nuclear transfer of somatic cell nuclei has been
extremely low, with less than 1% of transferred embryos surviving to term. Even the …
extremely low, with less than 1% of transferred embryos surviving to term. Even the …
A method for producing cloned pigs by using somatic cells as donors
L Lai, RS Prather - Germ Cell Protocols: Volume 2: Molecular Embryo …, 2004 - Springer
Nuclear transfer (NT) is a procedure by which genetically identical individuals can be
created. The applications of these NT techniques will be in agriculture, biomedicine, and …
created. The applications of these NT techniques will be in agriculture, biomedicine, and …
Improved efficiencies in the generation of multigene-modified pigs by recloning and using sows as the recipient
This study was performed to improve production efficiency at the level of recipient pig and
donor nuclei of transgenic cloned pigs used for xenotransplantation. To generate transgenic …
donor nuclei of transgenic cloned pigs used for xenotransplantation. To generate transgenic …
Effects of donor fibroblast cell type and transferred cloned embryo number on the efficiency of pig cloning
Z Li, J Shi, D Liu, R Zhou, H Zeng, X Zhou… - … " Cloning and Stem …, 2013 - liebertpub.com
Currently, cloning efficiency in pigs is very low. Donor cell type and number of cloned
embryos transferred to an individual surrogate are two major factors that affect the …
embryos transferred to an individual surrogate are two major factors that affect the …
Production of transgenic and non-transgenic clones in miniature pigs by somatic cell nuclear transfer
M Kurome, T Ishikawa, R Tomii, S Ueno… - … of Reproduction and …, 2008 - jstage.jst.go.jp
Miniature pigs have been recognized as valuable experimental animals in various fields
such as medical and pharmaceutical research. However, the amount of information on …
such as medical and pharmaceutical research. However, the amount of information on …
Influence of embryo handling and transfer method on pig cloning efficiency
J Shi, R Zhou, L Luo, R Mai, H Zeng, X He, D Liu… - Animal reproduction …, 2015 - Elsevier
The somatic cell nuclear transfer (SCNT) technique could be used to produce genetically
superior or genetically engineered cloned pigs that have wide application in agriculture and …
superior or genetically engineered cloned pigs that have wide application in agriculture and …