Development and validation of a highly efficient protocol of porcine somatic cloning using preovulatory embryo transfer in peripubertal gilts

B Petersen, A Lucas-Hahn, M Oropeza… - Cloning and stem …, 2008 - liebertpub.com
The efficiency of porcine somatic nuclear transfer (born piglets/transferred embryos) is low.
Here, we report a highly efficient protocol using peripubertal gilts as recipients synchronized …

Increasing efficiency in production of cloned piglets

H Callesen, Y Liu, HS Pedersen, R Li… - … (Formerly" Cloning and …, 2014 - liebertpub.com
The low efficiency in obtaining piglets after production of cloned embryos was challenged in
two steps—first by performing in vitro culture for 5–6 days after cloning to obtain later-stage …

Efficiency of porcine somatic cell nuclear transfer–a retrospective study of factors related to embryo recipient and embryos transferred

Y Huang, H Ouyang, H Yu, L Lai, D Pang… - Biology Open, 2013 - journals.biologists.com
The successful generation of pigs via somatic cell nuclear transfer depends on reducing risk
factors in several aspects. To provide an overview of some influencing factors related to …

Cloned Piglets Born after Nuclear Transplantation of Embryonic Blastomeres into Porcine Oocytes Matured In Vivo

GP Li, JH Tan, QY Sun, QG Meng, KZ Yue, XS Sun… - Cloning, 2000 - liebertpub.com
Nuclear transplantation in the pig is more difficult than in other domestic animals and only
one embryonic nuclear transplantation (NT) pig has been born to date. In this study …

A Highly Efficient Method for Porcine Cloning by Nuclear Transfer Using In Vitro-Matured Oocytes

SC Walker, T Shin, GM Zaunbrecher… - Cloning & Stem …, 2002 - liebertpub.com
To date, the efficiency of pig cloning by nuclear transfer of somatic cell nuclei has been
extremely low, with less than 1% of transferred embryos surviving to term. Even the …

A method for producing cloned pigs by using somatic cells as donors

L Lai, RS Prather - Germ Cell Protocols: Volume 2: Molecular Embryo …, 2004 - Springer
Nuclear transfer (NT) is a procedure by which genetically identical individuals can be
created. The applications of these NT techniques will be in agriculture, biomedicine, and …

Improved efficiencies in the generation of multigene-modified pigs by recloning and using sows as the recipient

J Cho, G Kim, AY Qamar, X Fang, PK Roy, BM Tanga… - Zygote, 2022 - cambridge.org
This study was performed to improve production efficiency at the level of recipient pig and
donor nuclei of transgenic cloned pigs used for xenotransplantation. To generate transgenic …

Effects of donor fibroblast cell type and transferred cloned embryo number on the efficiency of pig cloning

Z Li, J Shi, D Liu, R Zhou, H Zeng, X Zhou… - … " Cloning and Stem …, 2013 - liebertpub.com
Currently, cloning efficiency in pigs is very low. Donor cell type and number of cloned
embryos transferred to an individual surrogate are two major factors that affect the …

Production of transgenic and non-transgenic clones in miniature pigs by somatic cell nuclear transfer

M Kurome, T Ishikawa, R Tomii, S Ueno… - … of Reproduction and …, 2008 - jstage.jst.go.jp
Miniature pigs have been recognized as valuable experimental animals in various fields
such as medical and pharmaceutical research. However, the amount of information on …

Influence of embryo handling and transfer method on pig cloning efficiency

J Shi, R Zhou, L Luo, R Mai, H Zeng, X He, D Liu… - Animal reproduction …, 2015 - Elsevier
The somatic cell nuclear transfer (SCNT) technique could be used to produce genetically
superior or genetically engineered cloned pigs that have wide application in agriculture and …