[HTML][HTML] A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos
AF Ermisch, JR Herrick, R Pasquariello, MKC Dyer… - Scientific reports, 2020 - nature.com
Further refinement of culture media is needed to improve the quality of embryos generated
in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the …
in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the …
Mouse embryo cleavage, metabolism and viability: role of medium composition
DK Gardner, D Sakkas - Human Reproduction, 1993 - academic.oup.com
The cleavage, metabolism and viability of mouse zygotes were assessed after culture in
media of different ionic and metabolite composition. Medium with a high potassium …
media of different ionic and metabolite composition. Medium with a high potassium …
[HTML][HTML] Lipid-enriched reduced nutrient culture medium improves bovine blastocyst formation
R Pasquariello, M Zhang, JR Herrick… - Reproduction and …, 2023 - raf.bioscientifica.com
The refinement of embryo culture media is essential in improving embryo viability and in
vitro production efficiency. Our previous work demonstrated that the nutrients …
vitro production efficiency. Our previous work demonstrated that the nutrients …
[HTML][HTML] Developmental and molecular response of bovine embryos to reduced nutrients in vitro
JR Herrick, S Rajput, R Pasquariello… - Reproduction and …, 2020 - raf.bioscientifica.com
Recent studies in our laboratory have indicated that bovine embryos only use a small
amount of the nutrients available to them in culture. Our objective was to evaluate the …
amount of the nutrients available to them in culture. Our objective was to evaluate the …
[HTML][HTML] Systematic analysis of the factors that adversely affect the rate of cell accumulation in mouse embryos during their culture in vitro
XL Jin, C O'Neill - Reproductive Biology and Endocrinology, 2014 - Springer
Background Retarded embryo growth is a pervasive effect of culture in vitro. Methods A
systematic analysis of the interactions between media design, embryo culture density …
systematic analysis of the interactions between media design, embryo culture density …
[PDF][PDF] Development, glycolytic activity, and viability of preimplantation mouse embryos subjected to different periods of glucose starvation
G Leppens-Luisier, D Sakkas - Biology of reproduction, 1997 - scholar.archive.org
After compaction, the preimplantation mouse embryo switches to a glucose-based
metabolism, whereas for the 2-to 4-cell stage embryo, glucose can be inhibitory. In this …
metabolism, whereas for the 2-to 4-cell stage embryo, glucose can be inhibitory. In this …
The dynamic provision of different energy substrates improves development of one-cell random-bred mouse embryos in vitro
JJG Brown, DG Whittingham - Reproduction, 1992 - rep.bioscientifica.com
Preliminary observations showed that one-cell embryos from random-bred MF1 mice avoid
cleavage arrest at the two-cell stage ('in vitro two-cell block') when cultured in modified M16 …
cleavage arrest at the two-cell stage ('in vitro two-cell block') when cultured in modified M16 …
[HTML][HTML] Media optimization to promote rat embryonic development to the blastocyst stage in vitro
Efficient model production in rats that incorporates newly developed genetic editing and
embryo transfer tools, such as CRISPR/Cas9 technology and non-surgical embryo transfer …
embryo transfer tools, such as CRISPR/Cas9 technology and non-surgical embryo transfer …
Recent advances in the production of viable human embryos in vitro
TB Pool - Reproductive BioMedicine Online, 2002 - Elsevier
The introduction of stress to embryonic blastomeres through inappropriate culture conditions
results ultimately in the loss of viability. Retention of normal metabolic function in human …
results ultimately in the loss of viability. Retention of normal metabolic function in human …
[HTML][HTML] Composition of single-step media used for human embryo culture
DE Morbeck, NA Baumann, D Oglesbee - Fertility and sterility, 2017 - Elsevier
Objective To determine compositions of commercial single-step culture media and test with
a murine model whether differences in composition are biologically relevant. Design …
a murine model whether differences in composition are biologically relevant. Design …
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