Whole embryo culture (WEC) of postimplantation rodent embryos is widely used for the
study of mammalian embryogenesis and developmental toxicity testing. Its major advantage …

The aim of the present study was to formulate a simple chemically defined medium for the in
vitro growth of rat two-cell embryos to blastocysts. Embryos from day 2 pregnant rats were …

Since its development in the 1970's, whole embryo culture (WEC) has provided an important
method of growing and observing murine embryos ex utero. During WEC, embryos are …

Metabolism and epigenetics, which reciprocally regulate each other in different cell types,
are fundamental aspects of cellular adaptation to the environment. Evidence in cancer and …

Energy is essential for early embryogenesis, and fertilized eggs can successfully develop to
blastocyst in in vitro culture medium with an appropriate energy supply. Conversely …

Energy substrate requirements (pyruvate, lactate and amino acids) were determined for in-
vitro development of hamster 1-and 2-cell embryos to blastocysts, using a chemically …

BACKGROUND The majority of research aimed at improving embryo development in vitro
has focused on manipulation of the chemical environment, examining details such as …

Objective To determine if a culture medium, designated CZB after the authors who first
described it, which is supplemented with 0.11 mM ethylenediaminetetraacetic acid, 1.0 mM …

The effect of replacing 5.5 mM glucose in North Carolina State University (NCSU)-23
medium with 0.5 mM pyruvate/5.0 mM lactate on porcine IVF embryo development was …

Embryos are routinely cultured individually, although this can reduce blastocyst
development. Culture in atmospheric (20%) oxygen is also common, despite multiple …