Acinetobacter baylyi ADP1 as a model for metabolic system biology

V de Berardinis, M Durot, J Weissenbach… - Current opinion in …, 2009 - Elsevier
Information produced by the annotation of an 'average bacterial genome'can be separated
into three parts. One-third represents what we know, another third what we think we know …

Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium

V Barbe, D Vallenet, N Fonknechten… - Nucleic acids …, 2004 - academic.oup.com
Acinetobacter sp. strain ADP1 is a nutritionally versatile soil bacterium closely related to
representatives of the well-characterized Pseudomonas aeruginosa and Pseudomonas …

An Expanded Synthetic Biology Toolkit for Gene Expression Control in Acetobacteraceae

MY Teh, KH Ooi, SX Danny Teo… - ACS synthetic …, 2019 - ACS Publications
The availability of different host chassis will greatly expand the range of applications in
synthetic biology. Members of the Acetobacteraceae family of Gram-negative bacteria form …

Synthetic biology toolbox, including a single-plasmid CRISPR-Cas9 system to biologically engineer the electrogenic, metal-resistant bacterium Cupriavidus …

F Turco, M Garavaglia, R Van Houdt, P Hill… - ACS Synthetic …, 2022 - ACS Publications
Cupriavidus metallidurans CH34 exhibits extraordinary metabolic versatility, including
chemolithoautotrophic growth; degradation of BTEX (benzene, toluene, ethylbenzene …

Characterization and Modeling of Transcriptional Cross-Regulation in Acinetobacter baylyi ADP1

D Zhang, Y Zhao, Y He, Y Wang, Y Zhao… - Acs Synthetic …, 2012 - ACS Publications
Synthetic biology involves reprogramming and engineering of regulatory genes in
innovative ways for the implementation of novel tasks. Transcriptional gene regulation …

Iterative reconstruction of a global metabolic model of Acinetobacter baylyi ADP1 using high-throughput growth phenotype and gene essentiality data

M Durot, F Le Fèvre, V de Berardinis, A Kreimeyer… - BMC systems …, 2008 - Springer
Background Genome-scale metabolic models are powerful tools to study global properties
of metabolic networks. They provide a way to integrate various types of biological …

A complete collection of single‐gene deletion mutants of Acinetobacter baylyi ADP1

V De Berardinis, D Vallenet, V Castelli… - Molecular systems …, 2008 - embopress.org
We have constructed a collection of single‐gene deletion mutants for all dispensable genes
of the soil bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were …

High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration

JR Elmore, GN Dexter, H Baldino, JD Huenemann… - Science …, 2023 - science.org
Efficient genome engineering is critical to understand and use microbial functions. Despite
recent development of tools such as CRISPR-Cas gene editing, efficient integration of …

CRISPR interference–mediated metabolic engineering of Corynebacterium glutamicum for homo‐butyrate production

J Yoon, HM Woo - Biotechnology and bioengineering, 2018 - Wiley Online Library
Combinatorial metabolic engineering enabled the development of efficient microbial cell
factories for modulating gene expression to produce desired products. Here, we report the …

Genome-wide CRISPRi screen identifies enhanced autolithotrophic phenotypes in acetogenic bacterium Eubacterium limosum

J Shin, J Bae, H Lee, S Kang, S Jin… - Proceedings of the …, 2023 - National Acad Sciences
Acetogenic bacteria are a unique biocatalyst that highly promises to develop the sustainable
bioconversion of carbon oxides (eg, CO and CO2) into multicarbon biochemicals. Genotype …