The problem of mRNA decay in E. coli has recently seen exciting progress, with the
discoveries that key degradation enzymes are associated together in a high molecular …

Both end structures of eukaryotic mRNAs, namely the 5′ cap and 3′ poly (A) tail, are
necessary for transcript stability, and loss of either is sufficient to stimulate decay. mRNA …

THE degradation of messenger RNA in eukaryotic cells is initiated by endonucleolytic
cleavage1, 2 or by shortening of the poly (A) tail3–6, which for some mRNAs activates a …

While the potential importance of mRNA stability to the regulation of gene expression has
been recognized, the structures and mechanisms involved in the determination of individual …

Recognition of poly (A) sites in yeast pre‐mRNAs is poorly understood. Employing an in vitro
cleavage system with cleavage and polyadenylation factor (CPF) and cleavage factor IA we …

The process of mRNA decay is integral to the post-transcriptional control of gene
expression. The enzymes of the pathway have been identified, and now several laboratories …

Polyadenylation contributes to the destabilization of bacterial mRNA. We have investigated
the role of polyadenylation in the degradation of RNA by the purifiedEscherichia coli …

The addition of multiple adenosine residues to the 3'end of eukaryotic cell transcripts has a
key role in generating functional mRNA and in regulating the decay of this RNA (for recent …

We report that newly synthesized mRNA poly (A) tails are matured to precise lengths by the
Pab1p-dependent poly (A) nuclease (PAN) ofSaccharomyces cerevisiae. These results …

The major mRNA degradation pathway involves deadenylation of the target molecule
followed by decapping and, finally, 5′→ 3′ exonuclease digestion of the mRNA body …